B4GALT3 Recombinant Protein Antigen

Novus Biologicals | Catalog # NBP1-88653PEP

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Key Product Details

Source

E. coli

Tag

N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

Applications

Antibody Competition
View all B4GALT3 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human B4GALT3.

Source: E. coli

Amino Acid Sequence: YFGGVSALTPDQYLKMNGFPNEYWGWGGEDDDIATRVRLAGMKISRPPTSVGHYKMVKHRGDKGNEENPHRFDLLVRTQNSWTQDGMNSLTYQLLARELGPLYTNITADIGTDPRGPRAPSGPRYPPGS

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-88653.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation, and Storage

NBP1-88653PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: B4GALT3

Several oligosaccharide structures and protein glycoconjugate types are found in nature. Homologous glycosyltransferase (GT) gene families catalyze the formation of glycosidic linkages. The beta-1,3 galactosyltransferase(beta3GalT) gene family encodes a set of type II transmembrane glycoproteins that are catalytically diverse and use different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine ) to catalyze the addition of an activated monosaccharide to a terminal lactose. The protein coding sequences for beta-1,3-Gal-T genes comprise a single exon and are distantly related to the Drosophila Brainiac gene. The beta-1,4-galactosyltransferase (beta4GalT) gene family encodes type II membrane-bound glycoproteins that show exclusive specificity for the donor substrate, UDP-galactose. beta-1,4Gal-T genes transfer galactose in a beta-1,4 linkage to similar acceptor sugars; each gene has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures.

Alternate Names

b4Gal-T3, beta-1,4-galactosyltransferase 3, Beta-1,4-GalTase 3, beta4Gal-T3, beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 3, EC 2.4.1.-, UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3

Gene Symbol

B4GALT3

Additional B4GALT3 Products

Product Documents for B4GALT3 Recombinant Protein Antigen

Certificate of Analysis

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Product Specific Notices for B4GALT3 Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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