BAF57 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92966

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BAF57 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human BAF57 (NP_003070.3). MSKRPSYAPPPTPAPATQMPSTPGFVGYNPYSHLAYNNYRLGGNPGTNSRVTASSGITIPKPPKPPDKPLMPYMRYSRKVWDQVKASNPDLKLWEIGKIIGGMWRDLTDEEKQEYLNEYEAEKIEYNESMKAYHNSPAYLAYINAKSRAEAALEEESRQRQSRMEKGEPYMSIQPAEDPDDYDDGFSMKHTATARFQRNHRLISEILSESVVPDVRSVVTTARMQVLKRQVQSLMVHQRKLEAELLQIEERHQEKKRKFLESTDSFNNELKRLCGLKVEVDMEKIAAEIAQAEEQARKRQEEREKEAAEQAERSQSSIVPEEEQAANKGEEKKDDENIPMETEETHLEETTESQQNGEEGTSTPEDKESGQEGVDSMAEEGTSDSNTGSESNSATVEEPPTDPIPEDEKKE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BAF57 Antibody - BSA Free

Western Blot: BAF57 AntibodyBSA Free [NBP2-92966]

Western Blot: BAF57 AntibodyBSA Free [NBP2-92966]

Western Blot: BAF57 Antibody [NBP2-92966] - Western blot analysis of extracts from normal (control) and BAF57 knockout (KO) 293T cells, using BAF57 antibody (NBP2-92966) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunocytochemistry/ Immunofluorescence: BAF57 Antibody - BSA Free [NBP2-92966]

Immunocytochemistry/ Immunofluorescence: BAF57 Antibody - BSA Free [NBP2-92966]

Immunocytochemistry/Immunofluorescence: BAF57 Antibody [NBP2-92966] - Analysis of HeLa cells using BAF57.
Western Blot: BAF57 AntibodyBSA Free [NBP2-92966]

Western Blot: BAF57 AntibodyBSA Free [NBP2-92966]

Western Blot: BAF57 Antibody [NBP2-92966] - Analysis of extracts of various cell lines, using BAF57 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western Blot: BAF57 AntibodyBSA Free [NBP2-92966]

Western Blot: BAF57 AntibodyBSA Free [NBP2-92966]

Western Blot: BAF57 Antibody [NBP2-92966] - Analysis of 150ug extracts of Jurkat cells using BAF57 antibody. Western blot was performed from the immunoprecipitate using this antibody

Applications for BAF57 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

1:20-1:50

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BAF57

BAF57 is encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Alternate Names

BAF57BRG1-associated factor 57, chromatin remodeling complex BRG1-associated factor 57, subfamily e, member 1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily E member 1

Gene Symbol

SMARCE1

Additional BAF57 Products

Product Documents for BAF57 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BAF57 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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