Bag-1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56086

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Bag-1 Primary Antibodies »

Product Specifications

Immunogen

Full-length recombinant human BAG-1L protein was used as immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bag-1 Antibody - BSA Free

Simple Western: Bag-1 Antibody [NB100-56086]

Simple Western: Bag-1 Antibody [NB100-56086]

Simple Western: Bag-1 Antibody [NB100-56086] - Simple Western lane view shows a specific band for Bag-1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for Bag-1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:50-1:200

Simple Western

1:2500

Western Blot

1:1000-1:2000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:2500, apparent MW was 56 kDa.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bag-1

The BAG (Bcl-2-associated anthanogene) proteins are a family of chaperone regulators that modulate several diverse processes including proliferation, survival, stress responses, tumorigenesis, neuronal differentiation, growth arrest and apoptosis (reviewed Takayama and Reed, 2001; Doong et al, 2002, and Doukhanina et al. 2006). BAG proteins have been characterized as co-chaperones and interact with the chaperone heat shock proteins 70, both constitutive Hsc70 and inducible Hsp70. BAG proteins bind through their BAG domain to the ATPase domain of Hsc70/Hsp70 and can modulate either positively or negatively the functions of the Hsc70/Hsp70 chaperone proteins. The BAG domain has been shown to contribute to the anti-apoptotic activity of BAG- family proteins. The anti-apoptotic activities of BAG-family proteins may be dependent on their interactions with Hsc70/Asp70 and/or binding to Bcl-2. In addition to the conserved BAG domain, BAG-family proteins also contain additional domains which enable them to interact with specific target proteins or to target them to specific locations within cells. The BAG family contains at least six family members, including BAG-1 and its various isoforms [including BAG-1S, BAG-1M (Rap46/Hap46), and BAG-1L, BAG-2, BAG-3 (CAIR-1; Bis,), BAG-4 (SODD), BAG-5 and BAG-6 (Scythe, BAT3). The following amino acids lengths and molecular weights (kDa) have been described for human BAG proteins (reviewed in Takayama et al, 2001 and Doong et al, 2002): BAG-1 (230 amino acid, 34 kDa), BAG-1S (219 amino acid, 29 kDa), BAG-1M (274 amino acid, 46 kDa), BAG-1L (345 amino acid, 52 kDa), BAG-2 [212 amino acid; 24 kDa (Arndt et al. 2005)], BAG-3 (575 amino acid, 74 kDa), BAG-4 (456 amino acid; 60 kDa), BAG-5 ([442 amino acid; 51 kDa (Kalia et al. 2004)], and BAG-6 (1129 amino acid; 150 kDa). Recognizes both BAG-1 and BAG-1 isoforms.

Long Name

Bcl-2-associated Anthanogene-1

Alternate Names

Bag1

Entrez Gene IDs

573 (Human); 12017 (Mouse); 297994 (Rat)

Gene Symbol

BAG1

Additional Bag-1 Products

Product Documents for Bag-1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Bag-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

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