Rat BAK ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-69932

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.47 ng/mL

Assay Range

0.78 - 50 ng/mL
View all BAK ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat BAK1 in samples. No significant cross-reactivity or interference between Rat BAK1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.82%

Inter-Assay Precision (Precision between assays) CV% 4.58%

Recovery for Rat BAK ELISA Kit (Colorimetric)

Recovery

88-108%

Linearity

Rat BAK ELISA Kit (Colorimetric)

Scientific Data Images for Rat BAK ELISA Kit (Colorimetric)

Rat BAK ELISA Kit (Colorimetric)

ELISA: Rat BAK ELISA Kit (Colorimetric) [NBP2-69932] -

ELISA: Rat BAK ELISA Kit (Colorimetric) [NBP2-69932] - Standard Curve Reference

Kit Contents for Rat BAK ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: BAK

Bak a pro-apoptotic analog to Bax, is a member of the Bcl-2 family of proteins. During mitochondria-regulated programmed cell death, Bak promotes apoptosis by binding Bcl-2 and inhibiting its anti-apoptotic function (1). Upon apoptotic stimulation, Bak translocates from the cytosol to the mitochondrial membrane (2). Pro-apoptotic cascade activates BID, which oligomerizes Bak into pores that result in the release of cytochrome c (2, 3). In healthy cells, Bak associate with Bcl-xL and Mcl-1 but not with Bcl-2, Bcl-w or A1 and only when freed of both Bak will induce apoptosis.

Long Name

Bcl-2 Antagonist/Killer

Alternate Names

BAK1, BCL2L7

Gene Symbol

BAK1

Additional BAK Products

Product Documents for Rat BAK ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat BAK ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

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