Catalog # Availability Size / Price Qty
BDY FL, SE | CAS No. 146616-66-2 | Fluorescent Dyes
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Description: Green BDY (BODIPY® or boron-dipyrromethene) fluorescent dye for the labeling of amines; supplied as NHS ester

Chemical Name: (T-4)-[1-[3-[5-[(3,5-Dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-1H-pyrrol-2-yl-κN]-1-oxopropoxy]-2,5-pyrrolidinedionato]difluoroboron

Purity: ≥95%

Product Details

Biological Activity

BDY FL, SE is a fluorescent green BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Exhibits similar excitation and emission spectra to dyes TFAX 488 and FITC. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a C3 alkyl spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between fluorophore and conjugated biomolecule. The hydrophobic nature of BDY FL makes it ideal for labeling lipids and cell membranes. BDY FL also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~504 nM; emission maximum ~510 nM.

For more information on how BDY FL, SE may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.

It is recommended to prepare stock solutions in DMSO.

BODIPY® is a registered trademark of Molecular Probes.

Optical Data for BDY FL, SE

BDY FL, SE Dye Spectra
Emission Color Green
λabs 502 nm
λem 510 nm
Extinction Coefficient (ε) 80000 M-1cm-1
Quantum Yield (φ) 0.97
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable No
Correction Factor 260 0.015
Correction Factor 280 0.027
Application Confocal fluorescence, fluorescence polarization assays and two-photon excitation (TPE) microscopy
Alternative For FAM; Alexa Fluor 488; DyLight 488; Cy2

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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Technical Data

Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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