beta 2-Microglobulin Antibody (101F10) - BSA Free
Novus Biologicals | Catalog # NBP2-59477
![Western Blot: beta 2-Microglobulin Antibody (101F10)BSA Free [NBP2-59477]](https://beta-resources.rndsystems.com/images/products/beta-2-Microglobulin-Antibody-101F10-Western-Blot-NBP2-59477-img0001.jpg "Western Blot: beta 2-Microglobulin Antibody (101F10)BSA Free [NBP2-59477]")
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 101F10
Format
BSA Free
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Product Specifications
Immunogen
Recombinant human beta 2-Microglobulin (21-119aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Novus Biologicals Mouse beta 2-Microglobulin Antibody (101F10) - BSA Free (NBP2-59477) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for beta 2-Microglobulin Antibody (101F10) - BSA Free
Western Blot: beta 2-Microglobulin Antibody (101F10)BSA Free [NBP2-59477]
Western Blot: beta 2-Microglobulin Antibody (101F10) [NBP2-59477] - Lane 1: HeLa cell lysates, Lane 2: U937 cell lysates![Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (101F10) - BSA Free [NBP2-59477]](https://beta-resources.rndsystems.com/images/products/beta-2-Microglobulin-Antibody-101F10-Immunocytochemistry-Immunofluorescence-NBP2-59477-img0002.jpg "Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (101F10) - BSA Free [NBP2-59477]")
Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (101F10) - BSA Free [NBP2-59477]
Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (101F10) [NBP2-59477] - Analysis of B2M in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human B2M antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Applications for beta 2-Microglobulin Antibody (101F10) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1075
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: beta 2-Microglobulin
Alternate Names
B2M, beta 2Microglobulin
Gene Symbol
B2M
Additional beta 2-Microglobulin Products
Product Documents for beta 2-Microglobulin Antibody (101F10) - BSA Free
Product Specific Notices for beta 2-Microglobulin Antibody (101F10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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