beta Galactosidase Antibody

Novus Biologicals | Catalog # NBP3-50158

Novus Biologicals
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Key Product Details

Species Reactivity

E. coli

Applications

Western Blot, Dot Blot

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY
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Product Specifications

Immunogen

Prepared from eggs of chickens laid after repeated immunizations with full length native beta Galactosidase isolated from E.coli

Specificity

Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Description

Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against stated buffer.

Applications for beta Galactosidase Antibody

Application
Recommended Usage

Dot Blot

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody has been tested for use in ELISA and immunoblotting. Although not tested, this antibody may also be useful for neutralization assays, immunohistochemistry, flow Cytometry, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta Galactosidase

Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.

Alternate Names

EC 3.2.1.23; b-gal

Additional beta Galactosidase Products

Product Documents for beta Galactosidase Antibody

Certificate of Analysis

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Product Specific Notices for beta Galactosidase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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