beta-III Tubulin Antibody (24GB1740)

Novus Biologicals | Catalog # NBP3-51546

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB1740
View all beta-III Tubulin Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human beta-III Tubulin (Uniprot: Q13509)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for beta-III Tubulin Antibody (24GB1740)

beta-III Tubulin Antibody (24GB1740)

Flow Cytometry: beta-III Tubulin Antibody (24GB1740)[NBP3-51546] -

Validation of beta-III Tubulin knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with beta-III Tubulin antibody (1:2,000) and analyzed using BD flow cytometer.
beta-III Tubulin Antibody (24GB1740)

Flow Cytometry: beta-III Tubulin Antibody (24GB1740)[NBP3-51546] -

Flow cytometric analysis of beta-III Tubulinexpression in HT-1080 cells using beta-III Tubulin antibody (1:2,000). Green, isotype control; red, beta Tubulin III.
beta-III Tubulin Antibody (24GB1740)

Western Blot: beta-III Tubulin Antibody (24GB1740)[NBP3-51546] -

Western blotting analysis using beta-III Tubulin antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for beta-III Tubulin Antibody (24GB1740)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-III Tubulin

Beta-III Tubulin is abundant in the central and peripheral nervous systems where it is prominently expressed during fetal and postnatal development. Beta III Tubulin is often referred to as TUJ1 because Tuj-1 is the clone name of the original monoclonal antibody. The Tuj1 clone is such a popular neuronal marker that Tuj-1 is now used as a synonym for beta III tubulin. The distribution of Tubulin Beta 3 is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of the Tubulin Beta 3 protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers.

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III

Gene Symbol

TUBB3

Additional beta-III Tubulin Products

Product Documents for beta-III Tubulin Antibody (24GB1740)

Certificate of Analysis

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Download SDS

Product Specific Notices for beta-III Tubulin Antibody (24GB1740)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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