BI dihydrochloride

Tocris Bioscience | Catalog # 7466

DFHBI derivative for imaging of RNA in living cells that bind Broccoli aptamers
Tocris Bioscience

Key Product Details

Description

DFHBI derivative for imaging of RNA in living cells that bind Broccoli aptamers

Wavelength

Excitation = 470 nm, Emission = 505 nm
View all Light-up Aptamers »

Product Description

BI is a DFHBI derivative for imaging of RNA in cells. BI increases brightness and photostability of Broccoli aptamers in cells. BI enables imaging of single Broccoli-tagged mRNAs in living cells. BI exhibits peak excitation of 470 nm and peak fluorescence emission of 505 nm when bound to Broccoli. BI exhibits higher affinity for Broccoli aptamers and a 10.5-fold increase in cellular fluorescence compared with DFHBI-1T (Cat. No. 5610) (KD values are 51 and 305 nM, respectively).

Licensing Information

Sold under license from Lucerna

Optical Data

Emission Color Green
λabs 470 nm
λem 505 nm
Extinction Coefficient (ε) 33600 M-1cm-1
Quantum Yield (φ) 0.669
Cell Permeable Yes
Application DFHBI Derivative for Imaging of RNA in Living Cells

Spectra Viewer

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Product Specifications for BI dihydrochloride

Molecular Weight

441.26

Formula

C19H14F2N4O2.2HCl

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

(5Z)-3-(1H-Benzimidazol-7-ylmethyl)-5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-4H-imidazol-4-one dihydrochloride

CAS Number

2758438-31-0

InChI Key

YKXWKZLDMGAVJO-ZYMKCKPMSA-N

SMILES

CC1=N/C(C(N1CC2=C3NC=NC3=CC=C2)=O)=C\C4=CC(F)=C(C(F)=C4)O.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 44.13 100
DMSO 22.06 50

Preparing Stock Solutions for BI dihydrochloride

The following data is based on the product molecular weight 441.26.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.27 mL 11.33 mL 22.66 mL
5 mM 0.45 mL 2.27 mL 4.53 mL
10 mM 0.23 mL 1.13 mL 2.27 mL
50 mM 0.05 mL 0.23 mL 0.45 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for BI dihydrochloride

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for BI dihydrochloride

For research use only

Citations for BI dihydrochloride

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