Bim Antibody (3R0T2)

Novus Biologicals | Catalog # NBP3-15867

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3R0T2 expressed in HEK293
View all Bim Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bim (O43521). MAKQPSDVSSECDREGRQLQPAERPPQLRPGAPTSLQTEPQGNPEGNHGGEGDSCPHGSPQGPLAPPASPGPFATRSPLFIFMRRSSLLSRSSSGYFSFD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bim Antibody (3R0T2)

Western Blot: Bim Antibody (3R0T2) [NBP3-15867]

Western Blot: Bim Antibody (3R0T2) [NBP3-15867]

Western Blot: Bim Antibody (3R0T2) [NBP3-15867] - Western blot analysis of extracts of various cell lines, using Bim antibody (NBP3-15867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Western Blot: Bim Antibody (3R0T2) [NBP3-15867]

Western Blot: Bim Antibody (3R0T2) [NBP3-15867]

Western Blot: Bim Antibody (3R0T2) [NBP3-15867] - Western blot analysis of extracts of various cell lines, using Bim antibody (NBP3-15867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Applications for Bim Antibody (3R0T2)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bim

Bim, Bcl-2 interacting mediator of cell death, is a pro-apoptotic protein belonging to the

Alternate Names

BAM, bcl-2 interacting mediator of cell death, bcl-2 interacting protein Bim, Bcl2-interacting mediator of cell death, Bcl2-L-11, BCL2-like 11 (apoptosis facilitator), bcl-2-like protein 11, bcl-2-related ovarian death agonist, BIM-alpha6, BIM-beta6, BIM-beta7, BIMBOD, BimEL, BimL

Gene Symbol

BCL2L11

Additional Bim Products

Product Documents for Bim Antibody (3R0T2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Bim Antibody (3R0T2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Bim Antibody (3R0T2)

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Please recommend which isotype antibody I should use with the NBP1-75511AF647 (Bim-AF647.) The product datasheet only says IgG, but no subclass or product number.

    A: This item is a mouse IgG1 antibody. For isotype control, I would recommend NBP1-43319AF647 or NBP2-27287AF647.

  • Q: Would you recommend a standard Fix/Perm buffer for Bim staining?

    A:

    Our lab used our standard ICC protocol for cell staining with this product. We used tween-20 in the permeabilization step as BIM can be found in the endomembrane system and mitochondria.

  • Q: Please recommend which isotype antibody I should use with the NBP1-75511AF647 (Bim-AF647.) The product datasheet only says IgG, but no subclass or product number.

    A: This item is a mouse IgG1 antibody. For isotype control, I would recommend NBP1-43319AF647 or NBP2-27287AF647.

  • Q: Would you recommend a standard Fix/Perm buffer for Bim staining?

    A:

    Our lab used our standard ICC protocol for cell staining with this product. We used tween-20 in the permeabilization step as BIM can be found in the endomembrane system and mitochondria.

Showing  1 - 2 of 2 FAQs Showing All
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