Q: Please recommend which isotype antibody I should use with the NBP1-75511AF647 (Bim-AF647.) The product datasheet only says IgG, but no subclass or product number.

A: This item is a mouse IgG1 antibody. For isotype control, I would recommend NBP1-43319AF647 or NBP2-27287AF647.

Q: Would you recommend a standard Fix/Perm buffer for Bim staining?

A: Our lab used our standard ICC protocol for cell staining with this product. We used tween-20 in the permeabilization step as BIM can be found in the endomembrane system and mitochondria.

Human Bim ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-69950

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Product Documents

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human Bcl2L11 in samples. No significant cross-reactivity or interference between Human Bcl2L11 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.85%

Inter-Assay Precision (Precision between assays) CV% 5.12%

Recovery for Human Bim ELISA Kit (Colorimetric)

Recovery

84-108%

Linearity

Scientific Data Images for Human Bim ELISA Kit (Colorimetric)

ELISA: Human Bim ELISA Kit (Colorimetric) [NBP2-69950] -

ELISA: Human Bim ELISA Kit (Colorimetric) [NBP2-69950] - Standard Curve Reference

Kit Contents for Human Bim ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Bim

Bim, Bcl-2 interacting mediator of cell death, is a pro-apoptotic protein belonging to the

Alternate Names

BAM, bcl-2 interacting mediator of cell death, bcl-2 interacting protein Bim, Bcl2-interacting mediator of cell death, Bcl2-L-11, BCL2-like 11 (apoptosis facilitator), bcl-2-like protein 11, bcl-2-related ovarian death agonist, BIM-alpha6, BIM-beta6, BIM-beta7, BIMBOD, BimEL, BimL

Gene Symbol

BCL2L11

Additional Bim Products

Product Documents for Human Bim ELISA Kit (Colorimetric)

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Download Manual - Human Bim ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Human Bim ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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