Catalog Number: 3194
Alternate Names: 6BIO
Chemical Name: (2'Z,3'E)-6-Bromoindirubin-3'-oxime
Biological Activity
Potent and selective, ATP-competitive glycogen synthase kinase-3 (GSK-3) inhibitor (IC50 values are 5, 83, 300, 320 and > 10 000 nM at GSK-3, CDK5/p25, CDK2/cyclin A, CDK1/cyclin B and other common kinases respectively). Maintains self-renewal and pluripotency in embryonic stem cells via activation of the Wnt signaling pathway in vitro; also promotes proliferation and dedifferentiation in cardiomyocytes.

Negative Control also available.

Technical Data
  • M.Wt:
    356.17
  • Formula:
    C16H10BrN3O2
  • Solubility:
    Soluble to 10 mM in DMSO and to 10 mM in ethanol
  • Purity:
    >98%
  • Storage:
    Store at +4°C
  • CAS No:
    667463-62-9
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Additional Information
Other Product-Specific Information:
Background References
  1. The GSK-3 inhibitor BIO promotes proliferation in mammalian cardiomyocytes.
    Tseng et al.
    Chem.Biol., 2006;13:957
  2. GSK-3-selective inhibitors derived from tyrian purple indirubins.
    Meijer et al.
    Chem.Biol., 2003;10:1255
  3. Structural basis for the synthesis of indirubins as potent and selective inhibtors of glycogen synthase kinase-3 and cyclin dependent kinases.
    Polychronopoulos et al.
    J.Med.Chem., 2004;47:935
Citations:

The citations listed below are publications that use Tocris products. Selected citations for BIO include:

10 Citations: Showing 1 - 10
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  1. A High-content screen identifies compounds promoting the neuronal differentiation and the midbrain dopamine neuron specification of human neural progenitor cells.
    Authors: Rhim Et al.
    Elife 2015;5:16237
  2. A novel GSK3-regulated APC:Axin interaction regulates Wnt signaling by driving a catalytic cycle of efficient βcatenin destruction.
    Authors: Pronobis Et al.
    Cell 2015;4:e08022
  3. Rescue of an in vitro neuron phenotype identified in Niemann-Pick disease, type C1 induced pluripotent stem cell-derived neurons by modulating the WNT pathway and calcium signaling.
    Authors: Efthymiou Et al.
    Elife 2015;4:230
  4. Integrated β-catenin, BMP, PTEN, and Notch signalling patterns the nephron.
    Authors: Lindström Et al.
    Nat Chem Biol 2015;3:e04000
  5. GSK3 inhibitors stabilize Wee1 and reduce cerebellar granule cell progenitor proliferation.
    Authors: Penas Et al.
    Nat Commun 2015;14:417
  6. GSK3β-Dzip1-Rab8 cascade regulates ciliogenesis after mitosis.
    Authors: Zhang Et al.
    PLoS Biol 2015;13:e1002129
  7. GSK-3 modulates cellular responses to a broad spectrum of kinase inhibitors.
    Authors: Thorne Et al.
    Stem Cells Transl Med 2015;11:58
  8. Tissue-specific derepression of TCF/LEF controls the activity of the Wnt/β-catenin pathway.
    Authors: Lu Et al.
    Sci Rep 2014;5:5368
  9. PAK-dependent STAT5 serine phosphorylation is required for BCR-ABL-induced leukemogenesis.
    Authors: Berger Et al.
    Cell Cycle 2014;28:629
  10. Slug and Sox9 cooperatively determine the mammary stem cell state.
    Authors: Guo Et al.
    World J Gastroenterol 2012;148:1015
Tocris Small Molecules
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