Biotinyl Tyramide

Tocris Bioscience | Catalog # 6241

Reagent widely used for signal amplification in IHC and FISH
Tocris Bioscience

Key Product Details

Description

Reagent widely used for signal amplification in IHC and FISH
View all Tyramide Signaling Amplification/TSA »

Product Description

Biotinyl Tyramide is a reagent widely used for signal amplification in IHC and fluorescent in situ hybridization (ISH) and APEX proximity labeling. HRP catalyzes localized deposition of multiple biotinyl tyramide molecules (catalyzed reporter deposition, CARD), which can be detected using a labeled streptavidin conjugate. The localization of deposition around HRP leads to good resolution. Suitable for either fluorescence or chromogenic detection. Biotinyl Tyramide is used as a substrate for APEX proximity labeling.

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Product Specifications for Biotinyl Tyramide

Molecular Weight

363.47

Formula

C18H25N3O3S

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

(3aS,4S,6aR)-Hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide

CAS Number

41994-02-9

PubChem ID

21826423

InChI Key

VZWXNOBHWODXCW-ZOBUZTSGSA-N

SMILES

[H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCC3=CC=C(C=C3)O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 36.35 100

Preparing Stock Solutions for Biotinyl Tyramide

The following data is based on the product molecular weight 363.47.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.75 mL 13.76 mL 27.51 mL
5 mM 0.55 mL 2.75 mL 5.50 mL
10 mM 0.28 mL 1.38 mL 2.75 mL
50 mM 0.06 mL 0.28 mL 0.55 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 9 publications citing the usage of this product.

Product Documents for Biotinyl Tyramide

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Biotinyl Tyramide

For research use only

Citations for Biotinyl Tyramide

Customer Reviews for Biotinyl Tyramide (2)

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  • Biotinyl tyramide used successfully for BP-labelling of mitochondrial proteins
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 02/12/2020
    BP-labelling experiment in SH-SY5Y cells stably expressing MTS-APEX2. Tested range 500-1000µM biotinyl tyramide.
    Biotinyl Tyramide 6241
  • Great product for Biotinylation
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: SH-SY5Y
    Verified Customer | Posted 02/07/2020
    We use biotinyl tyramide to biotinylate cells - we have done this successfully without any issues to date. Biotinylated proteins can be detected by western blot using a streptavidin conjugated antibody (see image)
    Biotinyl Tyramide 6241

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