Bleomycin sulfate

Tocris Bioscience | Catalog # 7147

DNA synthesis inhibitor. Induces pulmonary inflammation and fibrosis
Tocris Bioscience

Key Product Details

Description

DNA synthesis inhibitor. Induces pulmonary inflammation and fibrosis

Alternative Names

NSC125066
View all DNA, RNA and Protein Synthesis Inhibitors »

Product Description

Bleomycin sulfate is a glycopeptide antibiotic produced by Streptomyces verticillus. It inhibits DNA synthesis and causes single and double-strand breaks at specific base sequences in intracellular DNA of bacterial and mammalian cells. It also acts as an inducer and regulator of apoptosis and inhibits tumor angiogenesis. It is an anticancer agent for squamous cell carcinomas (SCC) with IC50 = 4 nM in UT-SCC-19A cells.

In vivo, Bleomycin sulfate induces pulmonary inflammation and fibrosis in lung injury models (mice).

Bleomycin sulfate contains two main components:

1) Bleomycin A2 hemisulfate, C55H84N17O21S3.HSO4, molecular weight = 1512.62 Da

2) Bleomycin B2, C55H84N20O21S2, molecular weight = 1425.52 Da.

The exact proportions vary from batch to batch and can be found on the relevant certificate of analysis.

Product Specifications for Bleomycin sulfate

Molecular Weight

1512.60

Formula

C55H84N17O21S3.HSO4 (for A2)

Storage

Store at -20°C

Purity

≥90% (HPLC)

CAS Number

9041-93-4

PubChem ID

9812642

InChI Key

WUIABRMSWOKTOF-OYALTWQYSA-N

SMILES

O=S(O)([O-])=O.O=C(N[C@@H]([C@@H](O[C@H]1[C@@H](O[C@@H]2[C@@H](O)[C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O2)[C@@H](O)[C@H](O)[C@H](CO)O1)C3=CN=CN3)C(N[C@@H]([C@H]([C@@H](C(N[C@H](C(NCCC4=NC(C5=NC(C(NCCC[S+](C)C)=O)=CS5)=CS4)=O)[C@@H](C)O)=O)C)O)C)=O)C6=NC([C@@H](NC[C@@H](C(N)=O)N)CC(N)=O)=NC(N)=C6C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 20 14
DMSO 20 14

Preparing Stock Solutions for Bleomycin sulfate

The following data is based on the product molecular weight 1512.60.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.14 mM 4.72 mL 23.61 mL 47.22 mL
0.7 mM 0.94 mL 4.72 mL 9.44 mL
1.4 mM 0.47 mL 2.36 mL 4.72 mL
7 mM 0.09 mL 0.47 mL 0.94 mL

Calculators

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Product Documents for Bleomycin sulfate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Bleomycin sulfate

For research use only

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