Human BLNK ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-69972

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human BLNK in samples. No significant cross-reactivity or interference between Human BLNK and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.25%

Inter-Assay Precision (Precision between assays) CV% 4.38%

Recovery for Human BLNK ELISA Kit (Colorimetric)

Recovery

86-105%

Linearity

Human BLNK ELISA Kit (Colorimetric)

Scientific Data Images for Human BLNK ELISA Kit (Colorimetric)

Human BLNK ELISA Kit (Colorimetric)

ELISA: Human BLNK ELISA Kit (Colorimetric) [NBP2-69972] -

ELISA: Human BLNK ELISA Kit (Colorimetric) [NBP2-69972] - Standard Curve Reference

Kit Contents for Human BLNK ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: BLNK

B-cell linker protein (BLNK, also known as SLP65, BASH, and BCA) is a B cell adaptor that plays an important role in B cell development and in BCR-signal transduction. Since BLNK does not encode any intrinsic enzymatic activity, its function is to serve as a scaffold for assembling macromolecular complexes which includes enzymes (PLC gamma, Vav and Btk) and additional linker proteins (Grb2 and Nck) (1-3). In addition, BLNK binds Btk and is required for activation of the transcription factor NF-

Long Name

B-cell Linker Protein

Alternate Names

BASH, Ly57, SLP65

Gene Symbol

BLNK

Additional BLNK Products

Product Documents for Human BLNK ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human BLNK ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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