Boc-D(OMe)-FMK Inhibitor

Novus Biologicals | Catalog # NBP2-29395

Novus Biologicals
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Key Product Details

Applications

Block/Neutralize, Functional Assay

Product Specifications

Application Notes

Use in blocking/ neutralizing reported in scientific literature (PMID 25096578) 1. This inhibitor is designed as a methyl ester to facilitate cell permeability. If the intended use is on purified or recombinant enzymes, esterase should be added to generate the free carboxyl groups. Please contact us for more details.
2. For in-vivo or in-vitro experiments extending longer than 12 hrs, fresh inhibitor may have to be added to culture medium or injected into animals.

Formulation, Preparation, and Storage

Preparation Method

Molecular Weight: 263
Formula: C11H18FNO5
Mass Spec: M+1=468.2
TLC: Single-Spot (EtOAc: Hexane, 3.7) : Rf:0.5
NMR: All functional groups are present

Formulation

Form: White Semi-Solid
Make a stock solution of 5, 10 or 20 mM in high purity DMSO (>99.9%). T

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Boc-D(OMe)-FMK

Members of the caspase family play key roles in apoptosis and inflammation. BOC-D(OMe)-FMK is a cell-permeable broad-spectrum caspase peptide inhibitor that irreversibly binds to the catalytic site of caspases proteases, and inhibits caspase-mediated apoptosis by preventing the processing of pro-caspases to their active forms (reviewed in 1-3). The BOC-D(OMe)-FMK peptide is O-methylated in the P position providing enhanced stability and increased cell permeability. BOC-D(OMe)-FMK (BOC-D-FMK) is typically used in assays to inhibit apoptosis. BOC-D-FMK has been used in many different types of apoptosis assays and published using a number of model system. Users may want to consult the literature for additional information regarding applications for BOC-D-FMK. The following search string is recommended for identifying references in PubMed using the BOC-D-FMK peptide inhibitor : BOC-D-FMK or BOC-FMK or BOC-ASP-FMK.

Alternate Names

BOC-Asp(OMe)-FMK, BOC-D-FMK, BOC-D-FMK [BOC-Asp(OMe)-FMK, Boc-DOMe-FMK

Additional Boc-D(OMe)-FMK Products

Product Documents for Boc-D(OMe)-FMK Inhibitor

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Boc-D(OMe)-FMK Inhibitor

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

Citations for Boc-D(OMe)-FMK Inhibitor

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Protocols

View specific protocols for Boc-D(OMe)-FMK Inhibitor (NBP2-29395):

Product Handling - Assay Method (NBP2-29395):
Materials:
Dissolve 1mg of BOC-D-FMK in DMSO to get appropriate concentration:
190 ul DMSO = 20mM
381 ul DMSO = 10 mM
762 ul DMSO = 5 mM

Method:
Add 2 ul of above stock solutions to 1 ml of culture medium containing cells to give final DMSO concentration of 0.2%. Levels of DMSO above this may cause some cellular toxicity thus masking the effect of the ICE-protease inhibitors. 2 ul of 10 mM stock in 1 ml medium = 20 uM final BOC-D-FMK concentration.