Bovine Serum Albumin

Tocris Bioscience | Catalog # 5217

BSA (bovine serum albumin) is a blocking buffer for IHC, ICC, ELISA and Western blotting; used to block non-specific antibody binding. Commonly used in cell culture
Tocris Bioscience

Key Product Details

Description

BSA (bovine serum albumin) is a blocking buffer for IHC, ICC, ELISA and Western blotting; used to block non-specific antibody binding. Commonly used in cell culture
View all Blocking Buffers and Reagents »

Product Description

Bovine Serum Albumin (BSA) is a widely used blocking reagent for use in immunohistochemistry (IHC), immunocytochemistry (ICC), ELISAs and Western blotting. Used to block non-specific binding of antibodies. Bovine Serum Albumin can also be used as a protein concentration standard in Bradford assay for protein quantification. BSA is also used in cell culture.

Product Specifications for Bovine Serum Albumin

Storage

Store at +4°C

Purity

≥98%

CAS Number

9048-46-8

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solubility Soluble to 100mg/ml in water

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 121 publications citing the usage of this product.

There are currently no references for this product.

Product Documents for Bovine Serum Albumin

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Bovine Serum Albumin

For research use only

Citations for Bovine Serum Albumin

Customer Reviews for Bovine Serum Albumin (6)

4.7 out of 5
6 Customer Ratings
5 Stars
83%
4 Stars
0%
3 Stars
17%
2 Stars
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1 Stars
0%

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Showing  1 - 5 of 6 reviews Showing All
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  • test
    Name: Chi Ngo
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: AGS, MNK-1, MKN-45
    Verified Customer | Posted 09/06/2025
    5% BSA was used to block non-specific binding in immunocytochemistry.
  • BSA as blocking buffer
    Name: Anonymous
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: A7R5 vSMC
    Verified Customer | Posted 03/05/2023
    5% BSA diluted in PBS. 30 min incubation with blocking buffer at RT
    5% blocking buffer for immunoflorescent staining.
    Bovine Serum Albumin 5217
  • WB blocking buffer
    Name: Anonymous
    Species: Mink
    Assay Type: In Vitro
    Cell Line/Tissue: MV-1
    Verified Customer | Posted 05/04/2021
    I prepared a 3% BSA solution in TBS and used this to block my membranes prior to the addition of primary antibodies as well as my diluent for my secondary antibodies.
    Bovine Serum Albumin 5217
  • Blocking in Westerns
    Name: Anna Højer Øster
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Ngn3gfp
    Verified Customer | Posted 02/12/2021
    in the tc and western blotting
  • Very good
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: smooth muscle cell
    Verified Customer | Posted 03/28/2019
    We use this product for WB. It works very well.
    Bovine Serum Albumin 5217
  • Western blot
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Verified Customer | Posted 08/05/2018
    Used to block PVDF membranes for WB. Perfect performance. Works better than milk

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