Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
Novus Biologicals | Catalog # NB820-59657
![Western Blot: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]](https://resources.rndsystems.com/images/products/Mouse-Brain-Whole-Tissue-Lysate-Adult-Normal-Western-Blot-NB820-59657-img0005.jpg "Western Blot: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]")
Key Product Details
Species
Applications
Product Summary for Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
Product Specifications
Applications
Application Notes
Specificity
Type
Life Stage
Tissue
Protein State
Tissue Condition
Subcellular Fraction
Scientific Data Images for Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
Western Blot: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]
Western Blot: Mouse Brain Whole Tissue Lysate (Adult Normal) [NB820-59657] - Western blot analysis of GAPDH (NB300-323) using whole normal mouse brain lysate [NB820-59657].![SDS-PAGE: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]](https://resources.rndsystems.com/images/products/Mouse-Brain-Whole-Tissue-Lysate-Adult-Normal-SDS-Page-NB820-59657-img0004.jpg "SDS-PAGE: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]")
SDS-PAGE: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]
SDS-Page: Mouse Brain Whole Tissue Lysate (Adult Normal) [NB820-59657] - Ponceau S staining of whole normal mouse brain lysate [NB820-59657].![SDS-PAGE: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]](https://resources.rndsystems.com/images/products/Mouse-Brain-Whole-Tissue-Lysate-Whole-Normal-SDS-Page-NB820-59657-img0006.jpg "SDS-PAGE: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]")
SDS-PAGE: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]
SDS-Page: Mouse Brain Whole Tissue Lysate (Whole Normal) [NB820-59657] - Protein lysate is positively confirmed by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.Formulation, Preparation, and Storage
Formulation
Concentration
Shipping
Storage
Background: Brain
Additional Brain Products
Product Documents for Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
Certificate of Analysis
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Product Specific Notices for Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
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Q: Can this product be used as a positive control for anti-GluR2 (C-Term) and anti-GluR3 (N-term) antibodies?
A: We haven't tested these lysates for the specific expression of any single target, but I would expect GLUR2 and GLUR3 to be present. The only concern I have is how highly expressed these proteins would be in these samples and if they're abundant enough to serve as a good positive control. The donors for NB820-59657 vary, and you may see some change in signal if you were to probe multiple lots. I think that the best positive control (depending on your experiment) may be an over-expression lysate or recombinant protein.
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Q: We are seeking a normal brain tissue lysate for control of Parkinson’s disease. In your website, there is Human Brain Parietal Lobe Whole Tissue Lysate (Adult Parkinson's) 1.0mg (NB820-59414). If you have any normal brain tissue lysate, please let me know.
A: Please see this link to all of our normal human brain tissue lysates.
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Q: Can this product be used as a positive control for anti-GluR2 (C-Term) and anti-GluR3 (N-term) antibodies?
A: We haven't tested these lysates for the specific expression of any single target, but I would expect GLUR2 and GLUR3 to be present. The only concern I have is how highly expressed these proteins would be in these samples and if they're abundant enough to serve as a good positive control. The donors for NB820-59657 vary, and you may see some change in signal if you were to probe multiple lots. I think that the best positive control (depending on your experiment) may be an over-expression lysate or recombinant protein.
-
Q: We are seeking a normal brain tissue lysate for control of Parkinson’s disease. In your website, there is Human Brain Parietal Lobe Whole Tissue Lysate (Adult Parkinson's) 1.0mg (NB820-59414). If you have any normal brain tissue lysate, please let me know.
A: Please see this link to all of our normal human brain tissue lysates.