BRCA2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-30709
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
between 3350 and C-term
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit BRCA2 Antibody - BSA Free (NBP3-30709) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for BRCA2 Antibody - BSA Free
Immunoprecipitation:BRCA2 AntibodyNBP3-30709] -
Immunoprecipitation:BRCA2 AntibodyNBP3-30709] -Detection of human BRCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRCA2 antibody NBP3-30709 (lot NBP3-30709-4) used for IP at 6 µg per reaction. BRCA2 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-30709-3). For blotting immunoprecipitated BRCA2, NBP3-30709 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
BRCA2 AntibodyNBP3-30709] -
Whole cell lysate (50 µg) from HEK293T, OVCAR-3, HepG2, K-562, Jurkat, HeLa, U2OS, and MCF-7 cells preparedusing NETN lysis buffer. Antibody: Rabbit anti-BRCA2antibody for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposuretime of 3 minutes.Applications for BRCA2 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 µg/mg lysate
Western Blot
1:2,000 - 1:10,000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Tris-citrate/phosphate buffer, pH 7 to 8
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: BRCA2
Long Name
Breast Cancer 2
Alternate Names
BRCA1/BRCA2-containing complex, subunit 2, BRCC2, breast and ovarian cancer susceptibility gene, early onset, breast cancer 2 tumor suppressor, breast cancer 2, early onset, breast cancer susceptibility protein BRCA2, breast cancer type 2 susceptibility protein, BROVCA2, FACD, FAD, FAD1, FANCB, FANCD, FANCD1Fanconi anemia, complementation group D1, Fanconi anemia group D1 protein, GLM3, PNCA2
Gene Symbol
BRCA2
Additional BRCA2 Products
Product Documents for BRCA2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BRCA2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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