Human BRCA2 ELISA Kit (Chemiluminescence)

Novus Biologicals | Catalog # NBP2-70019

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

37.50 pg/mL

Assay Range

62.50-4000 pg/mL
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Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Species

Human

Specificity

This kit recognizes Human BRCA2 in samples. No significant cross-reactivity or interference between Human BRCA2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 10%

Inter-Assay Precision (Precision between assays) CV% 8.65%

Recovery for Human BRCA2 ELISA Kit (Chemiluminescence)

Recovery

88-101%

Linearity

Human BRCA2 ELISA Kit (Chemiluminescence)

Scientific Data Images for Human BRCA2 ELISA Kit (Chemiluminescence)

Human BRCA2 ELISA Kit (Chemiluminescence)

ELISA: Human BRCA2 ELISA Kit (Chemiluminescence) [NBP2-70019] -

ELISA: Human BRCA2 ELISA Kit (Chemiluminescence) [NBP2-70019] - Standard Curve Reference

Kit Contents for Human BRCA2 ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: BRCA2

BRCA 2 is localized to chromosome 13q12 - q13. Loss of heterozygosity of 13q is observed in 25% of sporadic breast tumors, which indicates that BRCA 2 might be a tumor suppressor gene. BRCA 2 confers only a low ovarian cancer risk. Reportedly, in MCF-10F (normal breast epithelial cell line) and MCF-7 (breast cancer cell line) cells the expression of BRCA 2 RNA lowers in G0 and early G1 phases then gets up-regulated at the G1 / S phase junction.

Long Name

Breast Cancer 2

Alternate Names

BRCA1/BRCA2-containing complex, subunit 2, BRCC2, breast and ovarian cancer susceptibility gene, early onset, breast cancer 2 tumor suppressor, breast cancer 2, early onset, breast cancer susceptibility protein BRCA2, breast cancer type 2 susceptibility protein, BROVCA2, FACD, FAD, FAD1, FANCB, FANCD, FANCD1Fanconi anemia, complementation group D1, Fanconi anemia group D1 protein, GLM3, PNCA2

Gene Symbol

BRCA2

Additional BRCA2 Products

Product Documents for Human BRCA2 ELISA Kit (Chemiluminescence)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human BRCA2 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Cancer Biomarkers

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