BRDG1/STAP-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-06491

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BRDG1 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human BRDG1/STAP-1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for BRDG1/STAP-1 Antibody - BSA Free

Western Blot: BRDG1/STAP-1 Antibody [NBP3-06491]

Western Blot: BRDG1/STAP-1 Antibody [NBP3-06491]

Western Blot: BRDG1/STAP-1 Antibody [NBP3-06491] - Lane A: Daudi Whole Cell LysateLane B: NCI-H460 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.)
Immunoprecipitation: BRDG1/STAP-1 Antibody [NBP3-06491]

Immunoprecipitation: BRDG1/STAP-1 Antibody [NBP3-06491]

Immunoprecipitation: BRDG1/STAP-1 Antibody [NBP3-06491] - Lane A:0.5 mg Daudi Whole Cell Lysate4 uL anti-STAP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-STAP1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :34 kDa

Applications for BRDG1/STAP-1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1-5ul/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRDG1

STAP-1 is encoded by this gene functions as a docking protein acting downstream of Tec tyrosine kinase in B cell antigen receptor signaling. The protein is directly phosphorylated by Tec in vitro where it participates in a postive feedback loop, increasing Tec activity. [provided by RefSeq]

Long Name

BCR Downstream-signaling Protein 1

Alternate Names

STAP1

Gene Symbol

STAP1

Additional BRDG1 Products

Product Documents for BRDG1/STAP-1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BRDG1/STAP-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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