Bromodeoxyuridine/BrdU Antibody (BRD/1539R)

Novus Biologicals | Catalog # NBP3-07317

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Non-species specific

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BRD/1539R
View all Bromodeoxyuridine/BrdU Primary Antibodies »

Product Specifications

Immunogen

Bromodeoxyuridine/BrdU conjugated to KLH

Localization

Nuclear

Marker

Proliferation Marker

Specificity

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

307 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08591)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for Bromodeoxyuridine/BrdU Antibody (BRD/1539R)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
ELISA: For coating, order antibody without BSA).
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Bromodeoxyuridine/BrdU

Bromodeoxyuridine (BrdU) is a synthetic nucleoside analogue of thymidine which can be incorporated into DNA in proliferating cells. During mitosis, BrdU may be incorporated into the newly synthezised DNA of S-phase cells, and can therefore provide an estimate for the fraction of cells in S-phase. Labeling cells with BrdU and then screening with BrdU antibodies has become a powerful tool to study the cytokinetics of normal and neoplastic cells. In vivo, BrdU is considered a potential carcinogen, as its incorporation into DNA may cause mutations.

Alternate Names

5-bromo-2-deoxyuridine, BrdU

Additional Bromodeoxyuridine/BrdU Products

Product Documents for Bromodeoxyuridine/BrdU Antibody (BRD/1539R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Bromodeoxyuridine/BrdU Antibody (BRD/1539R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle

Customer Reviews for Bromodeoxyuridine/BrdU Antibody (BRD/1539R)

There are currently no reviews for this product. Be the first to review Bromodeoxyuridine/BrdU Antibody (BRD/1539R) and earn rewards!

Have you used Bromodeoxyuridine/BrdU Antibody (BRD/1539R)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs for Bromodeoxyuridine/BrdU Antibody (BRD/1539R)

Showing  1 - 1 of 1 FAQ Showing All

  • Q: I have been using your BrdU antibody for some time now and saw that you have discontinued it. I was wondering if you had any alternative antibodies that would also work? it is antibody NB500-169 lot# 1706-5

    A:

    We recommend this product: NB500-235

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies