Catalog # Availability Size / Price Qty
Bumetanide | CAS No. 28395-03-1 | NKCC Cotransporter Inhibitors
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Description: Na+/2Cl-/K+ (NKCC) symporter inhibitor

Chemical Name: 3-(Aminosulfonyl)-5-(butylamino)-4-phenoxybenzoic acid

Purity: ≥98%

Product Details
Citations (12)
Supplemental Products

Biological Activity

Bumetanide is a loop diuretic that inhibits the Na+/2Cl-/K+ (NKCC) cotransporter. More potent than furosemide (Cat. No. 3109). Bumetanide improves electrophysiological, pathological and cognitive deficits in APOE4 knock-in mice. RNA sequencing of neurons derived from APOE4 induced pluripotent stem cell (iPSC) shows Bumetanide reverses Alzheimer's disease transcriptomic signatures.

Technical Data

Soluble to 100 mM in DMSO
Store at RT

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Background References

  1. Characteristics and functions of Na-K-Cl cotransport in epithelial tissues.
    O'Grady et al.
    Am.J.Physiol., 1987;253:C177
  2. Ion and bumet. binding by the Na-K-Cl cotransporter.
    Isenring and Forbush
    J.Biol.Chem., 1997;272:24556

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Citations for Bumetanide

The citations listed below are publications that use Tocris products. Selected citations for Bumetanide include:

12 Citations: Showing 1 - 10

  1. Purinergic signaling in cochlear supporting cells reduces hair cell excitability by increasing the extracellular space.
    Authors: Babola Et al.
    Elife  2020;9:e52160
  2. Toxoplasma-Induced Hypermigration of Primary Cortical Microglia Implicates GABAergic Signaling.
    Authors: Bhandage Et al.
    Front Cell Infect Microbiol  2019;9:73
  3. KCC2-Mediated Cl- Extrusion Modulates Spontaneous Hippocampal Network Events in Perinatal Rats and Mice.
    Authors: Spoljaric Et al.
    Cell Rep  2019;26:1073
  4. Cl- out is a novel cooperative optogenetic tool for extruding chloride from neurons
    Authors: Alfonsa Et al.
    Nature Communications  2016;7:13495
  5. Appearance of fast astrocytic component in voltage-sensitive dye imaging of neural activity.
    Authors: Pál Et al.
    Neuron  2015;8:35
  6. High salt intake increases blood pressure via BDNF-mediated downregulation of KCC2 and impaired baroreflex inhibition of vasopressin neurons.
    Authors: Choe Et al.
    Mol Brain  2015;85:549
  7. A role for intracellular zinc in glioma alteration of neuronal chloride equilibrium.
    Authors: Angelantonio Et al.
    Cell Death Dis  2014;5:e1501
  8. Disrupted Cl(-) homeostasis contributes to reductions in the inhibitory efficacy of D.pam during hyperexcited states.
    Authors: Deeb Et al.
    Eur J Neurosci  2013;38:2453
  9. GABA is excitatory in adult vasopressinergic neuroendocrine cells.
    Authors: Haam Et al.
    J Neurosci  2012;32:572
  10. The loop diuretic bumet. blocks posttraumatic p75NTR upregulation and rescues injured neurons.
    Authors: Shulga Et al.
    J Neurosci  2012;32:1757


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