Detects C. botulinum BoNT-G Light Chain in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant BoNT-A/LC, -B/LC, -C/LC, -D/LC, -E/LC, and BoNT-F/LC is observed.
Polyclonal Sheep IgG
E. coli-derived recombinant C. botulinum BoNT-G Light Chain Pro2-His427 Accession # Q60393
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of C. botulinum BoNT‑G Light Chain by Western Blot. Western blot shows recombinant C. botulinum BoNT-G Light Chain. PVDF membrane was probed with 1 µg/mL of Sheep Anti-C. botulinum BoNT‑G Light Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7079) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BoNT‑G Light Chain at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BoNT-G Light Chain
BoNT-G LC (Botulinum neurotoxin serotype G light chain) is a 47 kDa member of the peptidase M27 family of molecules. It is the product of Clostridium botulinum, and inhibits neurotransmitter release from the neuromuscular junction. This is accomplished by BoNT-G binding to synaptotagmin and ganglioside, followed by its internalization and subsequent light chain-mediated cleavage of synaptobrevin/VAMP that blocks synaptic vesicle fusion with the presynaptic membrane. BoNT-G precursor is 1297 amino acids (aa) in length. Following internalization and precursor proteolytic cleavage, it assumes a mature form that contains a 442 aa N-terminal enzymatic light chain disulfide-linked to an 855 aa C-terminal receptor-binding heavy chain. The 97 kDa heavy chain creates a channel within the endosome that allows for redox rupture of the disulfide bond and entry of the enzymatic light chain into the cytosol where it generates noncovalent homodimers. Over aa 2-427, BoNT-G light chain shares 60% aa identity with BoNT-B light chain.
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