C. botulinum BoNT-G Light Chain Antibody

    
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  • Species Reactivity
    C. botulinum
  • Specificity
    Detects C. botulinum BoNT-G Light Chain in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant BoNT-A/LC, -B/LC, -C/LC, -D/LC, -E/LC, and BoNT-F/LC is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant C. botulinum BoNT-G Light Chain
    Pro2-His427
    Accession # Q60393
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of C. botulinum BoNT‑G Light Chain by Western Blot. Western blot shows recombinant C. botulinum BoNT-G Light Chain. PVDF membrane was probed with 1 µg/mL of Sheep Anti-C. botulinum BoNT‑G Light Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7079) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BoNT‑G Light Chain at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BoNT-G Light Chain

BoNT-G LC (Botulinum neurotoxin serotype G light chain) is a 47 kDa member of the peptidase M27 family of molecules. It is the product of Clostridium botulinum, and inhibits neurotransmitter release from the neuromuscular junction. This is accomplished by BoNT-G binding to synaptotagmin and ganglioside, followed by its internalization and subsequent light chain-mediated cleavage of synaptobrevin/VAMP that blocks synaptic vesicle fusion with the presynaptic membrane. BoNT-G precursor is 1297 amino acids (aa) in length. Following internalization and precursor proteolytic cleavage, it assumes a mature form that contains a 442 aa N-terminal enzymatic light chain disulfide-linked to an 855 aa C-terminal receptor-binding heavy chain. The 97 kDa heavy chain creates a channel within the endosome that allows for redox rupture of the disulfide bond and entry of the enzymatic light chain into the cytosol where it generates noncovalent homodimers. Over aa 2-427, BoNT-G light chain shares 60% aa identity with BoNT-B light chain.

  • Long Name:
    Botulinum Neurotoxin Type G Light Chain
  • Alternate Names:
    BoNTG Light Chain; BoNT-G Light Chain
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 11
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB , Simple Western HAF016 17  
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL012 3
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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