CA125/MUC16 Antibody (OTI3C6) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-72337

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3C6

Format

Azide and BSA Free
View all CA125/MUC16 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 13942-14063 and 21117-21238 of human MUC16 (NP_078966) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for CA125/MUC16 Antibody (OTI3C6) - Azide and BSA Free

Western Blot: CA125/MUC16 Antibody (OTI3C6)Azide and BSA Free [NBP2-72337]

Western Blot: CA125/MUC16 Antibody (OTI3C6)Azide and BSA Free [NBP2-72337]

Western Blot: CA125/MUC16 Antibody (OTI3C6) - Azide and BSA Free [NBP2-72337] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MUC16 (SC200446, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted.

Applications for CA125/MUC16 Antibody (OTI3C6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CA125/MUC16

MUC16 (CA125) is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is over expressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller "subunits" being described by some investigators.

Long Name

Ovarian Cancer Antigen CA125

Alternate Names

MUC16

Gene Symbol

MUC16

Additional CA125/MUC16 Products

Product Documents for CA125/MUC16 Antibody (OTI3C6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CA125/MUC16 Antibody (OTI3C6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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