Catalog # Availability Size / Price Qty
Caffeine | CAS No. 58-08-2 | Non-selective Adenosine Receptor Antagonists
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Description: A1 and A2B antagonist. CNS stimulant

Chemical Name: 3,7-Dihydro-1,3,7-trimethyl-1H-purine-2,6-dione

Purity: ≥99%

Product Details
Citations (2)
Supplemental Products
Reviews (3)

Biological Activity

Caffeine is a central nervous system stimulant. Antagonist at A1 and A2A adenosine receptors and inhibitor of cyclic nucleotide phosphodiesterases. Mobilizes calcium from intracellular stores and inhibits benzodiazepine binding to GABA receptors.

Technical Data

Soluble to 100 mM in water and to 50 mM in DMSO
Store at RT

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Citations for Caffeine

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2 Citations: Showing 1 - 2


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Average Rating: 4.7 (Based on 3 Reviews)

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Low-dose oral caffeine induces a specific form of behavioral sensitization
By Kevin Ball on 01/28/2019
Application: Species: Rat

Rats had free access to untreated water or water containing 0.2 mg/ml of caffeine every other day of a 14-day experiment. This dose is relevant to human caffeine intake. Rats were tested on alternated days in an open-field arena for discrete movements (horizontal and vertical) and ambulatory distance. Results showed that although caffeine intake decreased significantly across test sessions, the number of discrete horizontal movements increased significantly, that is, showed sensitization.

PMID: 22358105 Reference

Caffeine acts on RyR2 in HL1 cells to release ER Ca2+.
By Anonymous on 12/16/2018
Application: Species: Mouse

HL-1 cells were loaded with cytosolic Ca2+ sensitive dye, Fura-2AM (1 µM)+F127 (0.01%) in normal tyrode solution for 40 min and excited alternately at 340 and 380 nm and fluorescence was captured at 510 nm. When the cells are perfused with 10 mM caffeine in nominally Ca2+ free tyrode solution, caffeine acts on RyR2 to release ER Ca2+, resulting in transient cytosolic peak (shown in the figure).

Caffeine as a tool to estimate sarcoplasmic Ca2+ content in cardiomyocytes
By Christopher Trummer on 10/15/2018
Application: Species: Other

Control or saxagliptin treated primary guinea pig cardiomyocytes were loaded with FURA-2AM (1 µM) and Pluronic F 127 (1%). Ca2+ transient decay tau evoked by caffeine (30 µM) is shown in the Image. Caffeine acts as a ryanodine receptor agonist and releases Ca2+ from sarcoplasmic reticulum into the cytosol.

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