Recombinant Human Calcineurin B His Protein

Name: Recombinant Human Calcineurin B His Protein
Brand: Novus Biologicals
SKU: NBP1-78797
Price: 999 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-78797

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all Calcineurin B Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-170 of Human Calcineurin B

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

21.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Calcineurin B His Protein

SDS-PAGE: Recombinant Human Calcineurin B His Protein [NBP1-78797]

SDS-Page: Recombinant Human Calcineurin B Protein [NBP1-78797] - 15% SDS-PAGE (3 ug).

Formulation, Preparation, and Storage

NBP1-78797

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2 mM DTT, 0.1 M NaCl
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calcineurin B

PPP3R1, also known as Calcineurin subunit B type 1, is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway. Calcineurin is composed of two subunits; calcineurin A (CnA) and calcineurin B (CnB). Dephosphorylation of the nuclear factor of activated T-cells (NF-AT) by Calcineurin is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. Recombinant human PPP3R1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

CALNB1, Cnb1, MCIP1, PP2B beta 1, PPP3R1

Gene Symbol

PPP3R1

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Product Documents for Recombinant Human Calcineurin B His Protein

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Product Specific Notices for Recombinant Human Calcineurin B His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.