Calnexin Antibody (1563) - Azide and BSA Free

Western Blot: Calnexin Antibody (1563) [NBP2-43765]

Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Analysis of A. 50 ug mouse liver lysate/extract 7.5 % SDS-PAGE Calnexin antibody [1563] dilution: 1:1000

Western Blot: Calnexin Antibody (1563) [NBP2-43765] -

Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (NBP2-43765) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Western Blot: Calnexin Antibody (1563) [NBP2-43765] -

Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (NBP2-43765) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Western Blot: Calnexin Antibody (1563) [NBP2-43765] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (15 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (NBP2-43765) diluted at 1:6000.

Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (1563) [NBP2-43765] -

Calnexin antibody [GT1563] detects Calnexin protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Calnexin stained by Calnexin antibody [GT1563] (NBP2-43765) diluted at 1:500.
Red: Calnexin, stained by Calnexin antibody [C3], C-term diluted at 1:500.
Blue: Hoechst 33342 staining.

Western Blot: Calnexin Antibody (1563) - Azide and BSA Free [NBP2-43765] -

AKT acts down-stream of mTOR signaling. To examine if Akt is upstream or downstream of mTOR signaling (A), osteoclast precursors were treated with RANKL (50 ng/ml) for 2–3 days with and without pyruvate (1 mM). (B) The levels of phosphorylated (Ser473) and total Akt in control and pyruvate-supplemented cultures, alpha -tubulin was used as a control for protein loading. (C) Cells cultured without pyruvate were exposed to Akt inhibitor (AI, 5 μM) or rapamycin (1, 10, or 100 nM), and mTOR phosphorylation, total mTOR levels and phosphorylation of p70S6K was assessed, calnexin was used as a protein loading control. The numbers above the blots indicate phospho-S6K levels relative to calnexin. (D) The effect of Akt inhibitor or rapamycin on Akt phosphorylation. The numbers above the blots indicate phospho-Akt levels relative to total Akt. To be noted, all the lanes were parts of the same gel, however, the lane order was changed, resulting in discontinuity. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28573133), licensed under a CC-BY license. Not internally tested by Novus Biologicals.