Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 3H4A7

Format

BSA Free
View all Calnexin Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide (CEAAEERPWLWVVYILTVAL) corresponding to a region of human Calnexin, conjugated to KLH. [UniProt# P27824]

Marker

Endoplasmic Reticulum Membrane Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calnexin Antibody (3H4A7) - BSA Free

Western Blot: Calnexin Antibody (3H4A7) [NBP1-47546]

Western Blot: Calnexin Antibody (3H4A7) [NBP1-47546]

Western Blot: Calnexin Antibody (3H4A7) [NBP1-47546] - Western blot analysis using anti-Calnexin monoclonal antibody against A431 (1), Hela (2), MCF-7 (3) and A549 (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (3H4A7) [NBP1-47546]

Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (3H4A7) [NBP1-47546]

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (3H4A7) [NBP1-47546] - Confocal immunofluorescence analysis of Hela cells using anti-Calnexin mAb (green).

Applications for Calnexin Antibody (3H4A7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This Calnexin (3H4A7) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and ELISA.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calnexin

Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatability complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP 78 and GRP 94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids). These features distinguish calnexin from soluble ER chaperones that cannot interact with the transmembrane and cytosolic domains of integral membrane proteins. The amino acid sequence of calnexin is highly conserved among species, and shares regions of high sequence homology with calreticulin.

Alternate Names

calnexin, CNX, IP90FLJ26570, Major histocompatibility complex class I antigen-binding protein p88, P90

Entrez Gene IDs

821 (Human)

Gene Symbol

CANX

UniProt

P27824 (Human)

Additional Calnexin Products

Product Documents for Calnexin Antibody (3H4A7) - BSA Free

Certificate of Analysis

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Product Specific Notices for Calnexin Antibody (3H4A7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Calnexin Antibody (3H4A7) - BSA Free

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Protocols

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