Calnexin Antibody [FITC]

Novus Biologicals | Catalog # NB100-1974F

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster, Zebrafish

Predicted:

Canine (90%), Orangutan (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG
View all Calnexin Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the rat Calnexin protein (between residues 550-591) [Uniprot: P35565]

Reactivity Notes

Hamster reactivity reported in the scientific literature (PMID: 23760268). Use in Zebrafish reported in scientific literature (PMID:23049555).

Localization

Endoplasmic reticulum

Marker

Endoplasmic Reticulum Membrane Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calnexin Antibody [FITC]

Calnexin Antibody [FITC]

Calnexin in U-2 OS Human Cell Line.

Calnexin was detected in immersion fixed U-2 OS human osteosarcoma cell line using Rabbit anti-Calnexin Affinity Purified Polyclonal Antibody conjugated to FITC (Catalog # NB100-1974F) (green) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for Calnexin Antibody [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Calnexin

Calnexin is a calcium-binding protein which interacts with newly synthesized glycoproteins in ER for facilitating protein assembly and/or the retention within ER of unassembled protein subunits as well as incorrectly folded proteins thereby playing a key role in ER's quality control apparatus. The specificity of calnexin for glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein. When associated with partial T-cell antigen receptor complexes which escape immature thymocytes's ER, calnexin regulates thymocyte maturation process and is also implicated in receptor-mediated endocytosis at synapses. MAPK3/ERK1 phosphorylates calnexin at Ser-565 leading to increased calnexin-ribosomal association and DHHC6 mediated calnexin palmitoylation leads to its preferential localization to perinuclear rough ER. Palmitoylation also facilitate calnexin association with RTC/ribosome-translocon complex that is essential to efficient folding of glycosylated proteins.

Alternate Names

calnexin, CNX, IP90FLJ26570, Major histocompatibility complex class I antigen-binding protein p88, P90

Gene Symbol

CANX

Additional Calnexin Products

Product Documents for Calnexin Antibody [FITC]

Certificate of Analysis

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Product Specific Notices for Calnexin Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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