Calpain 1 Antibody (SR2190) - BSA Free

Novus Biologicals | Catalog # NBP3-21569

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2190 expressed in HEK293

Format

BSA Free
View all Calpain 1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Calpain 1 (Uniprot #: P07384)

Specificity

Detects endogenous levels of total Calpain 1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calpain 1 Antibody (SR2190) - BSA Free

Calpain 1 Antibody (SR2190) - BSA Free

Western Blot: Calpain 1 Antibody (SR2190) - BSA Free [NBP3-21569] -

Western Blot: Calpain 1 Antibody (SR2190) - BSA Free [NBP3-21569] - Western blot analysis of Human fetal kidney lysate.

Applications for Calpain 1 Antibody (SR2190) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calpain 1

The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (Mu Calpain and M Calpain), a tissue specific calpain (N Calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Mu and M Calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa / 80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.

Long Name

Calpain-1 catalytic subunit

Alternate Names

Calpain mu-type, CANP 1, CANPL1, CAPN1, EC 3.4.22.52, Micromolar-calpain, muCANP, PIG30

Gene Symbol

CAPN1

Additional Calpain 1 Products

Product Documents for Calpain 1 Antibody (SR2190) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Calpain 1 Antibody (SR2190) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Calpain 1 Antibody (SR2190) - BSA Free

There are currently no reviews for this product. Be the first to review Calpain 1 Antibody (SR2190) - BSA Free and earn rewards!

Have you used Calpain 1 Antibody (SR2190) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies