Calpain 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15670

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Calpain 1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Chicken (80%), Rat (87%), Xenopus laevis (86%), Xenopus tropicalis (84%).

Localization

Cytoplasm; Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calpain 1 Antibody - BSA Free

Western Blot: Calpain 1 Antibody [NBP2-15670]

Western Blot: Calpain 1 Antibody [NBP2-15670]

Western Blot: Calpain 1 Antibody [NBP2-15670] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody [NBP2-15670]

Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody [NBP2-15670]

Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody [NBP2-15670] - Confocal immunofluorescence analysis of methanol-fixed A431, using Calpain 1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15670]

Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15670]

Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15670] - Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using antibody at 1:500 dilution.
Calpain 1 Antibody

Western Blot: Calpain 1 Antibody [NBP2-15670] -

Western Blot: Calpain 1 Antibody [NBP2-15670] - Sample (30 ug of whole cell lysate)
A: whole zebrafish
7.5% SDS PAGE
NBP2-15670 diluted at 1:1000
Calpain 1 Antibody

Immunohistochemistry: Calpain 1 Antibody [NBP2-15670] -

Immunohistochemistry: Calpain 1 Antibody [NBP2-15670] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Calpain 1 antibody (NBP2-15670) at 1:300 dilution.

Applications for Calpain 1 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Calpain 1

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq]

Long Name

Calpain-1 catalytic subunit

Alternate Names

Calpain mu-type, CANP 1, CANPL1, CAPN1, EC 3.4.22.52, Micromolar-calpain, muCANP, PIG30

Gene Symbol

CAPN1

UniProt

P07384 (Human)

Additional Calpain 1 Products

Product Documents for Calpain 1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Calpain 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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