Calsequestrin 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88181

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Calsequestrin 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LDFPEYDGVDRVINVNAKNYKNVFKKYEVLALLYHEPPEDDKASQRQFEMEELILELAAQVLEDKGVGFGLVDSEKDAAVAKKLGLTEVDSMYVFKGDEVIEYDGEFSADTIVEFLLDVLEDPVELIEGERELQAFENIEDEIKLIGY

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22093360)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calsequestrin 1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP1-88181]

Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP1-88181]

Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP1-88181] - Staining in human skeletal muscle and heart muscle tissues using anti-CASQ1 antibody. Corresponding CASQ1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP1-88181]

Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP1-88181]

Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP1-88181] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP1-88181]

Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP1-88181]

Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP1-88181] - Staining of human heart muscle shows low expression as expected.
Calsequestrin 1 Antibody - BSA Free Western Blot: Calsequestrin 1 Antibody - BSA Free [NBP1-88181]

Western Blot: Calsequestrin 1 Antibody - BSA Free [NBP1-88181]

Analysis in control (vector only transfected HEK293T lysate) and CASQ1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for Calsequestrin 1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calsequestrin 1

The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium.

Alternate Names

calmitin, calmitine, calsequestrin 1 (fast-twitch, skeletal muscle), Calsequestrin, skeletal muscle isoform, calsequestrin-1, CASQ, PDIB1

Gene Symbol

CASQ1

Additional Calsequestrin 1 Products

Product Documents for Calsequestrin 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Calsequestrin 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Calsequestrin 1 Antibody - BSA Free

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Protocols

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