CaM Kinase I Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-99439

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all CaM Kinase I Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Mouse CaM Kinase I.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CaM Kinase I Antibody - Azide and BSA Free

Western Blot: CaM Kinase I Antibody [NBP2-99439]

Western Blot: CaM Kinase I Antibody [NBP2-99439]

Western Blot: CaM Kinase I Antibody [NBP2-99439] - Anti-CaM Kinase I rabbit polyclonal antibody at 1:10000 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Mouse brain tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 44 kDa

Applications for CaM Kinase I Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:10000-1:50000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CaM Kinase I

Ca(+2)/calmodulin-dependent protein kinases (CaMKs) are activated upon binding of Ca(+2)/calmodulin. To gain maximal activity, CaMK I and CaMK IV can be further phosphorylated by an upstream kinase, CaMK kinase (CaMKK) (1). CaMKI is a Ca2+/calmodulin-dependent protein kinase that is widely expressed in eukaryotic cells and tissues but for which few, if any, physiological substrates are known (2). CaMKI has been shown to comprise a family of four structurally related isoforms (alpha, beta, gamma, and delta) encoded by separate genes with abundant expression in mature brain. In mouse brain, the four isoforms of CaMKI were found to exhibit distinct spatiotemporal expression during neuronal development. CaMKI may also have possible functional involvement of in the basal dendritic growth of hippocampal neurons (3)

Long Name

Calcium/Calmodulin-dependent Protein Kinase I

Alternate Names

calcium/calmodulin-dependent protein kinase I, calcium/calmodulin-dependent protein kinase type 1, CaM kinase I, CaM kinase I alpha, CaMKI, CaM-KI, caMKI-alpha, EC 2.7.11, EC 2.7.11.17, MGC120317, MGC120318

Gene Symbol

CAMK1

Additional CaM Kinase I Products

Product Documents for CaM Kinase I Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CaM Kinase I Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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