CaM Kinase II delta Antibody (10M1P2)

Novus Biologicals | Catalog # NBP3-16783

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10M1P2 expressed in HEK293
View all CaM Kinase II delta Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CaM Kinase II delta (Q13557). MASTTTCTRFTDEYQLFEELGKGAFSVVRRCMKIPTGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGFHYLVFDLVTGGELFE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CaM Kinase II delta Antibody (10M1P2)

Western Blot: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783]

Western Blot: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783]

Western Blot: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783] - Western blot analysis of extracts of various cell lines, using CaM Kinase II delta Rabbit mAb (NBP3-16783) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783]

Immunocytochemistry/ Immunofluorescence: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783]

Immunocytochemistry/Immunofluorescence: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783] - Immunofluorescence analysis of NIH-3T3 cells using CaM Kinase II delta Rabbit mAb (NBP3-16783) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783]

Immunocytochemistry/ Immunofluorescence: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783]

Immunocytochemistry/Immunofluorescence: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783] - Immunofluorescence analysis of C6 cells using CaM Kinase II delta Rabbit mAb (NBP3-16783) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
CaM Kinase II delta Antibody (10M1P2)

Immunocytochemistry/ Immunofluorescence: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783] -

Immunocytochemistry/ Immunofluorescence: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783] - Confocal imaging of NIH/3T3 cells using CaM Kinase II delta Rabbit mAb ollowed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for CaM Kinase II delta Antibody (10M1P2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CaM Kinase II delta

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Four alternatively spliced transcript variants that encode three different isoforms have been characterized to date. Distinct isoforms of this chain have different expression patterns.

Long Name

Calcium/calmodulin-dependent Protein Kinase II delta Subunit

Alternate Names

CAMK2D

Gene Symbol

CAMK2D

Additional CaM Kinase II delta Products

Product Documents for CaM Kinase II delta Antibody (10M1P2)

Certificate of Analysis

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Product Specific Notices for CaM Kinase II delta Antibody (10M1P2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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