cAMP Parameter Assay Kit (KGE002B/SKGE002B/PKGE002B): R&D Systems
  • Assay Length
    3.5 hours
  • Specificity
    Measures cyclic AMP levels in various samples
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC52, Parameter Immunoassay Control Set 764 for cAMP - Please Inquire
Product Summary
The Parameter cAMP Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure cAMP in cell culture supernates, cell lysates, plasma, serum, saliva, and urine.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean22.668.711621.770.4119
Standard Deviation1.372.552.542.267.3
CV%63.72.210.38.66.1

Cell Lysates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean18.556.996.11751.889.2
Standard Deviation0.891.742.731.33.56.9
CV%4.83.12.87.46.87.7

Recovery

The recovery of cAMP spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 104 86-119
Cell Lysis Buffer (n=1) 91 87-94
EDTA Plasma (n=4) 105 92-120
Heparin Plasma (n=4) 98 85-111
Saliva (n=4) 97 80-117
Serum (n=4) 103 97-114
Urine (n=6) 100 91-119
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of cAMP were serially diluted with the appropriate diluent to produce samples with values within the dynamic range of the assay.
cAMP Parameter Assay Kit
Preparation and Storage
  • Storage
    Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: cAMP

Adenosine 3,5-cyclic monophosphate (cAMP) is one of the most important intracellular second messengers. Several hormones are known to activate cAMP and subsequent signaling cascades via their indirect influence on adenylate cyclase, the enzyme responsible for the conversion of ATP to cAMP.

    • Long Name
      Cyclic AMP
    • Alternate Names
      cAMP;
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    54 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Protein kinase A-mediated phosphorylation of the Broad-Complex transcription factor in silkworm suppresses its transcriptional activity
      Authors: W Qian, X Gang, T Zhang, L Wei, X Yang, Z Li, Y Yang, L Song, P Wang, J Peng, D Cheng, Q Xia
      J. Biol. Chem., 2017;0(0):.
      Species: Silkworm
      Sample Type: Cell Culture Supernates
    2. BNGR-A25L and -A27 are two functional G protein-coupled receptors for CAPA periviscerokinin neuropeptides in the silkworm, Bombyx mori
      Authors: Z Shen, Y Chen, L Hong, Z Cui, H Yang, X He, Y Shi, L Shi, F Han, N Zhou
      J. Biol. Chem., 2017;0(0):.
      Species: Human
      Sample Type: Cell Lysates
    3. Membrane-Associated Effects of Glucocorticoid on BACE1 Upregulation and A? Generation: Involvement of Lipid Raft-Mediated CREB Activation
      Authors: GE Choi, SJ Lee, HJ Lee, SH Ko, CW Chae, HJ Han
      J. Neurosci., 2017;37(35):8459-8476.
      Species: Human
      Sample Type: Cell Lysates
    4. The neuropeptide F/nitric oxide pathway is essential for shaping locomotor plasticity underlying locust phase transition
      Authors: L Hou, P Yang, F Jiang, Q Liu, X Wang, L Kang
      Elife, 2017;6(0):.
      Species: Insect - Locust
      Sample Type: Tissue Homogenates
    5. Caffeine induces gastric acid secretion via bitter taste signaling in gastric parietal cells
      Authors: KI Liszt, JP Ley, B Lieder, M Behrens, V Stöger, A Reiner, CM Hochkogler, E Köck, A Marchiori, J Hans, S Widder, G Krammer, GJ Sanger, MM Somoza, W Meyerhof, V Somoza
      Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Endothelial PDGF-CC regulates angiogenesis-dependent thermogenesis in beige fat
      Nat Commun, 2016;7(0):12152.
      Species: Mouse
      Sample Type: Tissue Homogenates
    7. Functional Characterization of the Odorant Receptor 51E2 in Human Melanocytes
      J Biol Chem, 2016;0(0):.
      Species: Human
      Sample Type: Cell Lysates
    8. Molecular Characterization of E-Type Prostanoid Receptor 4 (EP4) from Ayu (Plecoglossus altivelis) and Its Functional Analysis in the Monocytes/Macrophages.
      Authors: Rong Y, Lu X, Chen J
      PLoS ONE, 2016;11(1):e0147884.
      Species: Fish - Plecoglossus altivelis (Ayu)
      Sample Type: Cell Lysates
    9. 5-HTR3 and 5-HTR4 located on the mitochondrial membrane and functionally regulated mitochondrial functions
      Sci Rep, 2016;6(0):37336.
      Species: Mouse
      Sample Type: Cell Lysates
    10. Sequence analysis and identification of new isoform of EP4 receptors in different atlantic salmon tissues (Salmo salar L.) and its role in PGE2 induced immunomodulation in vitro.
      Authors: Guo T, Gamil A, Koenig M, Evensen O
      PLoS ONE, 2015;10(3):e0120483.
      Species: Fish - Atlantic Salmon
      Sample Type: Cell Lysates
    11. beta-Hydroxybutyric sodium salt inhibition of growth hormone and prolactin secretion via the cAMP/PKA/CREB and AMPK signaling pathways in dairy cow anterior pituitary cells.
      Authors: Fu S, Wang W, Liu B, Yang H, Ji H, Yang Z, Guo B, Liu J, Wang J
      Int J Mol Sci, 2015;16(2):4265-80.
      Species: Bovine
      Sample Type: Cell Culture Supernates
    12. ASM-024, a piperazinium compound, promotes the in vitro relaxation of beta2-adrenoreceptor desensitized tracheas.
      Authors: Israel-Assayag E, Beaulieu M, Cormier Y
      PLoS ONE, 2015;10(3):e0120095.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Formaldehyde Crosses the Human Placenta and Affects Human Trophoblast Differentiation and Hormonal Functions.
      Authors: Pidoux G, Gerbaud P, Guibourdenche J, Therond P, Ferreira F, Simasotchi C, Evain-Brion D, Gil S
      PLoS ONE, 2015;10(7):e0133506.
      Species: Human
      Sample Type: Whole Cells
    14. Evaluating the A-Subunit of the Heat-Labile Toxin (LT) As an Immunogen and a Protective Antigen Against Enterotoxigenic Escherichia coli (ETEC).
      Authors: Norton E, Branco L, Clements J
      PLoS ONE, 2015;10(8):e0136302.
      Species: Human
      Sample Type: Cell Lysates
    15. The stress response neuropeptide CRF increases amyloid-beta production by regulating gamma-secretase activity.
      Authors: Park H, Ran Y, Jung J, Holmes O, Price A, Smithson L, Ceballos-Diaz C, Han C, Wolfe M, Daaka Y, Ryabinin A, Kim S, Hauger R, Golde T, Felsenstein K
      EMBO J, 2015;34(12):1674-86.
      Species: Human
      Sample Type: Cell Lysates
    16. Intermittent PTHrP(1-34) exposure augments chondrogenesis and reduces hypertrophy of mesenchymal stromal cells.
      Authors: Fischer, Jennifer, Aulmann, Antje, Dexheimer, Verena, Grossner, Tobias, Richter, Wiltrud
      Stem Cells Dev, 2014;23(20):2513-23.
      Species: Human
      Sample Type: Cell Culture Supernates
    17. Exemestane blocks mesothelioma growth through downregulation of cAMP, pCREB and CD44 implicating new treatment option in patients affected by this disease.
      Authors: Nuvoli, Barbara, Germoni, Sabrina, Morosetti, Carlotta, Santoro, Raffaela, Cortese, Giancarl, Masi, Serena, Cordone, Iole, Galati, Rossella
      Mol Cancer, 2014;13(0):69.
      Species: Human
      Sample Type: Cell Lysates
    18. Apremilast is a selective PDE4 inhibitor with regulatory effects on innate immunity.
      Authors: Schafer P, Parton A, Capone L, Cedzik D, Brady H, Evans J, Man H, Muller G, Stirling D, Chopra R
      Cell Signal, 2014;26(9):2016-29.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. Cannabidiol exerts sebostatic and antiinflammatory effects on human sebocytes.
      Authors: Olah A, Toth B, Borbiro I, Sugawara K, Szollosi A, Czifra G, Pal B, Ambrus L, Kloepper J, Camera E, Ludovici M, Picardo M, Voets T, Zouboulis C, Paus R, Biro T
      J Clin Invest, 2014;124(9):3713-24.
      Species: Human
      Sample Type: Cell Lysates
    20. Constitutive Galphai coupling activity of very large G protein-coupled receptor 1 (VLGR1) and its regulation by PDZD7 protein.
      Authors: Hu Q, Dong J, Du H, Zhang D, Ren H, Ma M, Cai Y, Zhao T, Yin X, Yu X, Xue T, Xu Z, Sun J
      J Biol Chem, 2014;289(35):24215-25.
      Species: Human
      Sample Type: Cell Culture Supernates
    21. Low adiposity during early infancy is associated with a low risk for developing dengue hemorrhagic fever: a preliminary model.
      Authors: Libraty D, Zhang L, Woda M, Giaya K, Kathivu C, Acosta L, Tallo V, Segubre-Mercado E, Bautista A, Obcena A, Brion J, Capeding R
      PLoS ONE, 2014;9(2):e88944.
      Species: Human
      Sample Type: Cell Culture Supernates
    22. Cardiac progenitor cells engineered with betaARKct have enhanced beta-adrenergic tolerance.
      Authors: Khan M, Mohsin S, Toko H, Alkatib M, Nguyen J, Truffa S, Gude N, Chuprun K, Tilley D, Koch W, Sussman M
      Mol Ther, 2014;22(1):178-85.
      Species: Human
      Sample Type: Cell Lysates
    23. Antidiarrheal efficacy and cellular mechanisms of a Thai herbal remedy.
      Authors: Tradtrantip L, Ko E, Verkman A
      PLoS Negl Trop Dis, 2014;8(2):e2674.
      Species: Human
      Sample Type: Cell Culture Supernates
    24. Simultaneous exposure to Escherichia coli heat-labile and heat-stable enterotoxins increases fluid secretion and alters cyclic nucleotide and cytokine production by intestinal epithelial cells.
      Authors: Read L, Hahn R, Thompson C, Bauer D, Norton E, Clements J
      Infect Immun, 2014;82(12):5308-16.
      Species: Human
      Sample Type: Cell Lysates
    25. An siRNA screen identifies the GNAS locus as a driver in 20q amplified breast cancer.
      Authors: Garcia-Murillas I, Sharpe R, Pearson A, Campbell J, Natrajan R, Ashworth A, Turner N
      Oncogene, 2014;33(19):2478-86.
    26. Activation of glucagon-like peptide-1 receptor inhibits growth and promotes apoptosis of human pancreatic cancer cells in a cAMP-dependent manner.
      Authors: Zhao H, Wei R, Wang L, Tian Q, Tao M, Ke J, Liu Y, Hou W, Zhang L, Yang J, Hong T
      Am J Physiol Endocrinol Metab, 2014;306(12):E1431-41.
      Species: Human
      Sample Type: Whole Cells
    27. Specific activation of the G protein-coupled receptor BNGR-A21 by the neuropeptide corazonin from the silkworm, Bombyx mori, dually couples to the G(q) and G(s) signaling cascades.
      Authors: Yang, Jingwen, Huang, Haishan, Yang, Huipeng, He, Xiaobai, Jiang, Xue, Shi, Ying, Alatangaole, Damirin, Shi, Liangen, Zhou, Naiming
      J Biol Chem, 2013;288(17):11662-75.
      Species: Human
      Sample Type: Cell Culture Supernates
    28. Bioengineered 3D human kidney tissue, a platform for the determination of nephrotoxicity.
      Authors: DesRochers, Teresa M, Suter, Laura, Roth, Adrian, Kaplan, David L
      PLoS ONE, 2013;8(3):e59219.
      Species: Human
      Sample Type: Cell Culture Supernates
    29. Role of tyrosine phosphorylation in the muscarinic activation of the cystic fibrosis transmembrane conductance regulator (CFTR).
      Authors: Billet A, Luo Y, Balghi H, Hanrahan J
      J Biol Chem, 2013;288(30):21815-23.
      Species: Hamster
      Sample Type: Cell Lysates
    30. Amyloid beta (Abeta) peptide directly activates amylin-3 receptor subtype by triggering multiple intracellular signaling pathways.
      Authors: Fu W, Ruangkittisakul A, MacTavish D, Shi JY, Ballanyi K, Jhamandas JH
      J. Biol. Chem., 2012;287(22):18820-30.
      Species: Human
      Sample Type: Cell Lysates
    31. Hypoxia and prostaglandin e receptor 4 signalling pathways synergise to promote endometrial adenocarcinoma cell proliferation and tumour growth.
      Authors: Catalano RD, Wilson MR, Boddy SC
      PLoS ONE, 2011;6(5):e19209.
      Species: Human
      Sample Type: Cell Lysates
    32. beta1-Adrenergic receptors activate two distinct signaling pathways in striatal neurons.
      Authors: Meitzen J, Luoma JI, Stern CM, Mermelstein PG
      J. Neurochem., 2011;116(6):984-95.
      Species: Rat
      Sample Type: Cell Lysates
    33. Cross-Reactivity of Anthrax and C2 Toxin: Protective Antigen Promotes the Uptake of Botulinum C2I Toxin into Human Endothelial Cells.
      Authors: Kronhardt A, Rolando M, Beitzinger C, Stefani C, Leuber M, Flatau G, Popoff MR, Benz R, Lemichez E
      PLoS ONE, 2011;6(8):e23133.
      Species: Human
      Sample Type: Cell Lysates
    34. Glucagon-like peptide-1 (GLP-1) and the regulation of human invariant natural killer T cells: lessons from obesity, diabetes and psoriasis.
      Authors: Hogan AE, Tobin AM, Ahern T, Corrigan MA, Gaoatswe G, Jackson R, O'Reilly V, Lynch L, Doherty DG, Moynagh PN, Kirby B, O'Connell J, O'Shea D
      Diabetologia, 2011;54(11):2745-54.
      Species: Human
      Sample Type: Cell Lysates
    35. gammadeltaT-cell function in sepsis is modulated by C5a receptor signalling.
      Authors: Han G, Geng S, Li Y, Chen G, Wang R, Li X, Ma Y, Shen B, Li Y
      Immunology, 2011;133(3):340-9.
      Species: Mouse
      Sample Type: Cell Lysates
    36. Anti-inflammatory role of microsomal prostaglandin E synthase-1 in a model of neuroinflammation.
      Authors: Brenneis C, Coste O, Altenrath K, Angioni C, Schmidt H, Schuh CD, Zhang DD, Henke M, Weigert A, Brune B, Rubin B, Nusing R, Scholich K, Geisslinger G
      J. Biol. Chem., 2011;286(3):2331-42.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    37. Transforming growth factor beta1 inhibits cystic fibrosis transmembrane conductance regulator-dependent cAMP-stimulated alveolar epithelial fluid transport via a phosphatidylinositol 3-kinase-dependent mechanism.
      Authors: Roux J, Carles M, Koh H, Goolaerts A, Ganter MT, Chesebro BB, Howard M, Houseman BT, Finkbeiner W, Shokat KM, Paquet AC, Matthay MA, Pittet JF
      J. Biol. Chem., 2010;285(7):4278-90.
      Species: Rat
      Sample Type: Cell Lysates
    38. EP2 receptor mediated cAMP release is augmented by PGF 2 alpha activation of the FP receptor via the calcium-calmodulin pathway.
      Authors: Abera AB, Sales KJ, Catalano RD, Katz AA, Jabbour HN
      Cell. Signal., 2010;22(1):71-9.
      Species: Human
      Sample Type: Cell Lysates
    39. Mechanism of glucocorticoid protection of airway smooth muscle from proasthmatic effects of long-acting beta2-adrenoceptor agonist exposure.
      Authors: Nino G, Hu A, Grunstein JS, Grunstein MM
      J. Allergy Clin. Immunol., 2010;125(5):1020-7.
      Species: Human
      Sample Type: Cell Lysates
    40. Differential expression and functional characterization of luteinizing hormone receptor splice variants in human luteal cells: implications for luteolysis.
      Authors: Dickinson RE, Stewart AJ, Myers M, Millar RP, Duncan WC
      Endocrinology, 2009;150(6):2873-81.
      Species: Human
      Sample Type: Cell Lysates
    41. Reduced adiposity and high-fat diet-induced adipose inflammation in mice deficient for phosphodiesterase 4B.
      Authors: Zhang R, Maratos-Flier E, Flier JS
      Endocrinology, 2009;150(7):3076-82.
      Species: Mouse
      Sample Type: Cell Lysates
    42. Human female hair follicles are a direct, nonclassical target for thyroid-stimulating hormone.
      Authors: Bodo E, Kromminga A, Bíró T, Borbiro I, Gáspár E, Zmijewski MA, van Beek N, Langbein L, Slominski AT, Paus R
      J. Invest. Dermatol., 2009;129(5):1126-39.
      Species: Human
      Sample Type: Cell Culture Supernates
    43. Thiophenecarboxylate suppressor of cyclic nucleotides discovered in a small-molecule screen blocks toxin-induced intestinal fluid secretion.
      Authors: Tradtrantip L, Yangthara B, Padmawar P, Morrison C, Verkman AS
      Mol. Pharmacol., 2009;75(1):134-42.
      Species: Canine
      Sample Type: Cell Lysates
    44. Exendin-4 has an anti-hypertensive effect in salt-sensitive mice model.
      Authors: Hirata K, Kume S, Araki S, Sakaguchi M, Chin-Kanasaki M, Isshiki K, Sugimoto T, Nishiyama A, Koya D, Haneda M, Kashiwagi A, Uzu T
      Biochem. Biophys. Res. Commun., 2009;380(1):44-9.
      Species: Mouse
      Sample Type: Urine
    45. Rational combined targeting of phosphodiesterase 4B and SYK in DLBCL.
      Authors: Kim SW, Rai D, McKeller MR, Aguiar RC
      Blood, 2009;113(24):6153-60.
      Species: Human
      Sample Type: Cell Lysates
    46. Mechanism regulating proasthmatic effects of prolonged homologous beta2-adrenergic receptor desensitization in airway smooth muscle.
      Authors: Nino G, Hu A, Grunstein JS, Grunstein MM
      Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(4):L746-57.
      Species: Human
      Sample Type: Cell Lysates
    47. Long term oncostatin M treatment induces an osteocyte-like differentiation on osteosarcoma and calvaria cells.
      Authors: Brounais B, David E, Chipoy C, Trichet V, Ferre V, Charrier C, Duplomb L, Berreur M, Redini F, Heymann D, Blanchard F
      Bone, 2009;44(5):830-9.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    48. Inhibition of interleukin-1beta-induced matrix metalloproteinases 1 and 13 production in human osteoarthritic chondrocytes by prostaglandin D2.
      Authors: Zayed N, Afif H, Chabane N, Mfuna-Endam L, Benderdour M, Martel-Pelletier J, Pelletier JP, Motiani RK, Trebak M, Duval N, Fahmi H
      Arthritis Rheum., 2008;58(11):3530-40.
      Species: Human
      Sample Type: Cell Lysates
    49. Prostaglandin E2 stimulates Fas ligand expression via the EP1 receptor in colon cancer cells.
      Authors: O'Callaghan G, Kelly J, Shanahan F, Houston A
      Br. J. Cancer, 2008;99(3):502-12.
      Species: Human
      Sample Type: Cell Culture Supernates
    50. Morphine withdrawal inhibits IL-12 induction in a macrophage cell line through a mechanism that involves cAMP.
      Authors: Kelschenbach J, Ninkovic J, Wang J, Krishnan A, Charboneau R, Barke RA, Roy S
      J. Immunol., 2008;180(6):3670-9.
      Species: Mouse
      Sample Type: Cell Lysates
    51. Dehydroepiandrosterone stimulates phosphorylation of FoxO1 in vascular endothelial cells via phosphatidylinositol 3-kinase- and protein kinase A-dependent signaling pathways to regulate ET-1 synthesis and secretion.
      Authors: Chen H, Lin AS, Li Y, Reiter CE, Ver MR, Quon MJ
      J. Biol. Chem., 2008;283(43):29228-38.
      Species: Bovine
      Sample Type: Cell Lysates
    52. CT20126, a novel immunosuppressant, prevents collagen-induced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation.
      Authors: Lee SJ, Nam WD, Na HJ, Cho YL, Ha KS, Hwang JY, Lee H, Kim SO, Kwon YG, Kim YM
      Biochem. Pharmacol., 2008;76(1):79-90.
      Species: Human
      Sample Type: Cell Culture Supernates
    53. Acylated and unacylated ghrelin promote proliferation and inhibit apoptosis of pancreatic beta-cells and human islets: involvement of 3',5'-cyclic adenosine monophosphate/protein kinase A, extracellular signal-regulated kinase 1/2, and phosphatidyl inositol 3-Kinase/Akt signaling.
      Authors: Granata R, Settanni F, Biancone L, Trovato L, Nano R, Bertuzzi F, Destefanis S, Annunziata M, Martinetti M, Catapano F, Ghè C, Isgaard J, Papotti M, Ghigo E, Muccioli G
      Endocrinology, 2007;148(2):512-29.
      Species: Hamster
      Sample Type: Cell Lysates
    Expand to show all 54 Citations
    ELISA Controls
    Description Application Cat# Citations Images  

    Parameter Immunoassay Control Set 764 for cAMP

    Ctrl QC52
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....

    Have you used cAMP Parameter Assay Kit?

    Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Order Details

    Contact Information

    R&D Systems Guarantee

    Customers Who Viewed
    This Item Also Viewed

    Feedback

    Customer Information