CAMSAP1L1 Antibody

Novus Biologicals | Catalog # NBP1-21402

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Mouse, Rat

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CAMSAP1L1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1439 and 1489 of human calmodulin regulated spectrin-associated protein 1-like 1 using the numbering given in entry EAW91320.1 (GeneID 23271).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24440982)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CAMSAP1L1 Antibody

Western Blot: CAMSAP1L1 Antibody [NBP1-21402]

Western Blot: CAMSAP1L1 Antibody [NBP1-21402]

CAMSAP1L1-Antibody-Western-Blot-NBP1-21402-img0004.jpg
Western Blot: CAMSAP1L1 Antibody [NBP1-21402]

Western Blot: CAMSAP1L1 Antibody [NBP1-21402]

Western Blot: CAMSAP1L1 Antibody [NBP1-21402] - on HeLa whole cell lysate using anti-CAMSAP1L1 antibody. CAMSAP1L1 was also immunoprecipitated by rabbit anti-CAMSAP1L1 antibody NBP1-21401.
Knockdown Validated: CAMSAP1L1 Antibody [NBP1-21402]

Immunocytochemistry/ Immunofluorescence: CAMSAP1L1 Antibody [NBP1-21402]

CAMSAP1L1-Antibody-Knockdown-Validated-NBP1-21402-img0003.jpg
CAMSAP1L1 Antibody

Immunocytochemistry/ Immunofluorescence: CAMSAP1L1 Antibody [NBP1-21402] -

Immunocytochemistry/ Immunofluorescence: CAMSAP1L1 Antibody [NBP1-21402] - Loss of non-centrosomal MTs has a more severe impact than their detachment from the Golgi in 3D but not in 2D.(A) Staining for CAMSAP2 (white, green) & Golgi (GM130, red) in HUVECs transfected with indicated siRNA. The plot shows CAMSAP2 enrichment at the Golgi (ratio between the average CAMSAP2 intensity on top of Golgi & in cytoplasm), n = 20 cells per condition. Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/33864), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CAMSAP1L1 Antibody

Immunocytochemistry/ Immunofluorescence: CAMSAP1L1 Antibody [NBP1-21402] -

Immunocytochemistry/ Immunofluorescence: CAMSAP1L1 Antibody [NBP1-21402] - CAMSAP2 is required for maintaining non-centrosomal MTs & cell migration in ECs.(G) Staining for CAMSAP2 (white/green), gamma -tubulin (red) & DNA (DAPI, blue) in HUVECs. Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/33864), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CAMSAP1L1 Antibody

Western Blot: CAMSAP1L1 Antibody [NBP1-21402] -

Western Blot: CAMSAP1L1 Antibody [NBP1-21402] - The centrosome is not essential for angiogenic migration & sprouting.(A) WBs of total extract from HUVECs treated or not treated with centrinone used to quantify the levels of alpha -tubulin & CAMSAP2 protein expression as well as of tubulin post-translational modifications (acetylated, detyrosinated & polyglutamylated tubulin); Ku80 antibody used as loading control, n = 3 independent experiments & n = 4 for CAMSAP2. Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/33864), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CAMSAP1L1 Antibody

Western Blot: CAMSAP1L1 Antibody [NBP1-21402] -

Western Blot: CAMSAP1L1 Antibody [NBP1-21402] - CAMSAP2 is required for maintaining non-centrosomal MTs & cell migration in ECs. (E) WBs of extracts of HUVECs treated as in (D) used to quantify the levels of alpha -tubulin & CAMSAP2 expression & the levels of tubulin post-translational modifications (acetylated, detyrosinated & polyglutamylated tubulin); Ku80 antibody used as loading control, n = 3 independent experiments. Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/33864), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CAMSAP1L1 Antibody

Immunocytochemistry/ Immunofluorescence: CAMSAP1L1 Antibody [NBP1-21402] -

Immunocytochemistry/ Immunofluorescence: CAMSAP1L1 Antibody [NBP1-21402] - CAMSAP2 participates in Golgi polarization during 2D migration & 3D sprouting. (C) Staining for CAMSAP2 (white/green), Golgi (GM130, red), F-actin (phalloidin, white) & DNA (DAPI, blue) in HUVEC spheroid sprouting 14 hr after 3D spheroid embedding. Z-maximum projections of confocal images shown; arrows show polarized Golgi co-localizing with CAMSAP2 stretches & the dashed line indicates the position of spheroid body. Data shown using box plots; Student’s unpaired two-tailed t-test (A), Mann-Whitney U test (B): *p<0.05, ns, no significant difference.10.7554/eLife.33864.028Figure 5—figure supplement 1—source data 1.An Excel sheet with numerical data on the quantification of effect of CAMSAP2 depletion on the polarization of centrosome during migration & the speed, duration & length of Rab6 tracks in 2D migrating ECs represented as plots in Figure 5—figure supplement 1A,B.An Excel sheet with numerical data on the quantification of effect of CAMSAP2 depletion on the polarization of centrosome during migration & the speed, duration & length of Rab6 tracks in 2D migrating ECs represented as plots in Figure 5—figure supplement 1A,B. Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/33864), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CAMSAP1L1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID 24440982)

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Reviewed Applications

Read 1 review rated 4 using NBP1-21402 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CAMSAP1L1

CAMSAP1L1 (calmodulin-regulated spectrin-associated protein 1-like protein 1) is related to CAMSAP1. Both contain an N-terminal CH (calponin homology domain) which is an actin-binding domain found in cytoskeletal and signal transduction proteins. The CAMSAP1 family of proteins also contains a unique C-terminal CKK (DUF1781) domain which has been found to bind microtubules. The function of CAMSAP1L1 has not been characterized. CAMSAP1L1 is also known as KIAA7078.

Alternate Names

calmodulin regulated spectrin-associated protein 1-like 1, calmodulin-regulated spectrin-associated protein 2, CAMSAP2, KIAA1078Calmodulin-regulated spectrin-associated protein 1-like protein 1, MGC150680, MGC150681

Entrez Gene IDs

23271 (Human)

Gene Symbol

CAMSAP2

UniProt

Q08AD1 (Human)

Additional CAMSAP1L1 Products

Product Documents for CAMSAP1L1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CAMSAP1L1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CAMSAP1L1 Antibody

Customer Reviews for CAMSAP1L1 Antibody (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used CAMSAP1L1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • CAMSAP1L1 Antibody
    Name: Kung-Hsien Ho
    Application: Western Blot
    Sample Tested: Min6 mouse insulinoma whole cell lysate
    Species: Mouse
    Verified Customer | Posted 10/05/2017
    Western blot against CAMSAP2 (wm 168KD) from MIN6 cells transduced with or without Lentivirus-CAMSAP2-shRNA. 6% acryamide gel
    Lane 1: Protein ladder (250 - 150 - 100 - 75) Lane 2: untransduced MIN6. Lane 3-6: Lentivirus-CAMSAP2-shRNA transduced MIN6 (virus titration left to right, low to high).
    CAMSAP1L1 Antibody NBP1-21402

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies