Canine IL-17/IL-17A Quantikine ELISA Kit

Name: Canine IL-17/IL-17A Quantikine ELISA Kit
Brand: R&D Systems
SKU: CA1700

R&D Systems | Catalog # CA1700

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Product Documents

Please consider catalog # DY5848 as an alternative.

Discontinued Product

CA1700 has been discontinued. View all IL-17/IL-17A products.

Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)

Sensitivity

9.2 pg/mL

Assay Range

31.3-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

View all IL-17/IL-17A ELISA Kits »

Product Summary for Canine IL-17/IL-17A Quantikine ELISA Kit

The Quantikine Canine IL-17A Immunoassay is a 4.5 hour solid-phase ELISA designed to measure canine IL-17A in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant canine IL-17A and antibodies raised against the recombinant protein. Results obtained for naturally occurring canine IL-17A showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine IL-17A.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Canine

Specificity

Natural and recombinant canine IL-17A.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	111	257	855	107	238	797
Standard Deviation	2.90	8.65	26.5	8.16	14.1	41.7
CV%	2.6	3.4	3.1	7.6	5.9	5.2

Recovery for Canine IL-17/IL-17A Quantikine ELISA Kit

The recovery of canine IL-17A spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	102	85-112
EDTA Plasma (n=4)	91	82-102
Heparin Plasma (n=4)	89	79-103
Serum (n=4)	93	85-102

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of canine IL-17A were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Images for Canine IL-17/IL-17A Quantikine ELISA Kit

Canine IL-17/IL-17A Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17/IL-17A

IL-17/IL-17A is a homodimeric cytokine that is secreted by IL-23 activated Th17 and Tc17 cells. It is upregulated during inflammation and contributes to disease severity by enhancing neutrophil infiltration and the production of pro-inflammatory chemokines. IL-17A can additionally form bioactive heterodimers with IL-17F. IL-17A and the IL-17A/F heterodimer signal through widely expressed receptor complexes containing IL-17 RA and IL-17 RC.