Canine IL-17/IL-17A Quantikine ELISA Kit: R&D Systems

Canine IL-17/IL-17A Quantikine ELISA Kit

CA1700 has been discontinued. View all IL-17/IL-17A products.
Please consider catalog # DY5848 as an alternative.
  
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    9.2 pg/mL
  • Assay Range
    31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant canine IL-17A.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Canine IL-17A Immunoassay is a 4.5 hour solid-phase ELISA designed to measure canine IL-17A in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant canine IL-17A and antibodies raised against the recombinant protein. Results obtained for naturally occurring canine IL-17A showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine IL-17A.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean111257855107238797
Standard Deviation2.98.6526.58.1614.141.7
CV%2.63.43.17.65.95.2

Recovery

The recovery of canine IL-17A spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 85-112
EDTA Plasma (n=4) 91 82-102
Heparin Plasma (n=4) 89 79-103
Serum (n=4) 93 85-102
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of canine IL-17A were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Canine IL-17/IL-17A Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-17/IL-17A
The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.
    • Long Name
      Interleukin 17
    • Entrez Gene IDs
      3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine);
    • Alternate Names
      CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A;
    Related Research Areas

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