carabin Antibody

Novus Biologicals | Catalog # NBP2-76349

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence C-PRRKPQTRGKTFH corresponding to internal region according to NP_940919.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for carabin Antibody

Western Blot: carabin Antibody [NBP2-76349]

Western Blot: carabin Antibody [NBP2-76349]

Western Blot: carabin Antibody [NBP2-76349] - Staining of Human Spleen (A) and Mouse Thymus (B) lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.
Flow Cytometry: carabin Antibody [NBP2-76349]

Flow Cytometry: carabin Antibody [NBP2-76349]

Flow Cytometry: carabin Antibody [NBP2-76349] - Paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: carabin Antibody [NBP2-76349]

Western Blot: carabin Antibody [NBP2-76349]

Western Blot: carabin Antibody [NBP2-76349] - Staining of Jurkat with antibody at 0.5 ug/mL (A), PBM with antibody at 0.3 ug/mL (B) and negative control A431 (C) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Applications for carabin Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:28000
Application Notes
WB: Preliminary experiments in human tonsil, thymus and peripheral mononucleocytes lysates gave no specific signal but low background.

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Advanced Features

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: carabin

Carabin is an endogenous inhibitor of calcineurin (see MIM 114105) that also inhibits the Ras (see MIM 190020) signaling pathway through its intrinsic Ras GTPase-activating protein activity (Pan et al., 2007 [PubMed 17230191]).[supplied by OMIM]

Alternate Names

carabin, FLJ00332, MGC46488, TBC1 domain family member 10C, TBC1 domain family, member 10C

Gene Symbol

TBC1D10C

Additional carabin Products

Product Documents for carabin Antibody

Certificate of Analysis

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Product Specific Notices for carabin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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