Carbonic Anhydrase VA/CA5A Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92181

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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Carbonic Anhydrase VA/CA5A Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 39-305 of human CA5A (NP_001730.1). CAWQTSNNTLHPLWTVPVSVPGGTRQSPINIQWRDSVYDPQLKPLRVSYEAASCLYIWNTGYLFQVEFDDATEASGISGGPLENHYRLKQFHFHWGAVNEGGSEHTVDGHAYPAELHLVHWNSVKYQNYKEAVVGENGLAVIGVFLKLGAHHQTLQRLVDILPEIKHKDARAAMRPFDPSTLLPTCWDYWTYAGSLTTPPLTESVTWIIQKEPVEVAPSQLSAFRTLLFSALGEEEKMMVNNYRPLQPLMNRKVWASFQATNEGTRS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Carbonic Anhydrase VA/CA5A Antibody - Azide and BSA Free (NBP2-92181) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Carbonic Anhydrase VA/CA5A Antibody - Azide and BSA Free

Carbonic Anhydrase VA/CA5A Antibody - Azide and BSA Free

Western Blot: Carbonic Anhydrase VA/CA5A Antibody - Azide and BSA Free [NBP2-92181] -

Western Blot: Carbonic Anhydrase VA/CA5A Antibody - Azide and BSA Free [NBP2-92181] - Western blot analysis of lysates from Mouse liver using Carbonic Anhydrase VA/CA5A Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time:5s.
Carbonic Anhydrase VA/CA5A Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase VA/CA5A Antibody - Azide and BSA Free [NBP2-92181] -

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase VA/CA5A Antibody - Azide and BSA Free [NBP2-92181] - Immunofluorescence analysis of NIH/3T3 cells using Carbonic Anhydrase VA/CA5A Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Carbonic Anhydrase VA/CA5A Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase VA/CA5A Antibody - Azide and BSA Free [NBP2-92181] -

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase VA/CA5A Antibody - Azide and BSA Free [NBP2-92181] - Immunofluorescence analysis of PC-12 cells using Carbonic Anhydrase VA/CA5A Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for Carbonic Anhydrase VA/CA5A Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase VA/CA5A

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VA is localized in the mitochondria and expressed primarily in the liver. It may play an important role in ureagenesis and gluconeogenesis. CA5A gene maps to chromosome 16q24.3 and an unprocessed pseudogene has been assigned to 16p12-p11.2. [provided by RefSeq]

Alternate Names

CA5A, CAVA

Gene Symbol

CA5A

Additional Carbonic Anhydrase VA/CA5A Products

Product Documents for Carbonic Anhydrase VA/CA5A Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Carbonic Anhydrase VA/CA5A Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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