Carbonic Anhydrase XIV/CA14 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87477

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Carbonic Anhydrase XIV/CA14 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GQHWTYEGPHGQDHWPASYPECGNNAQSPIDIQTDSVTFDPDLPALQPHGYDQPGTEPLDLHNNGHTVQLSLPSTLYLGGLPRKYVAAQLHLHWGQKGSPGGSEHQINSEATFAELHIVHYDSDSYDSLSEAAERPQGLAVLGIL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Carbonic Anhydrase XIV/CA14 Antibody - BSA Free

Western Blot: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477]

Western Blot: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477]

Western Blot: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477] - Analysis in control (vector only transfected HEK293T lysate) and CA14 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477]

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477]

Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477] - Staining of human cell line SK-MEL-30 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Applications for Carbonic Anhydrase XIV/CA14 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase XIV/CA14

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA XIV is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. [provided by RefSeq]

Alternate Names

CA14

Gene Symbol

CA14

Additional Carbonic Anhydrase XIV/CA14 Products

Product Documents for Carbonic Anhydrase XIV/CA14 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Carbonic Anhydrase XIV/CA14 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Carbonic Anhydrase XIV/CA14 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review Carbonic Anhydrase XIV/CA14 Antibody - BSA Free and earn rewards!

Have you used Carbonic Anhydrase XIV/CA14 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies