Carboxypeptidase B1/CPB1 Antibody Pair [HRP]

Novus Biologicals | Catalog # NBP2-79570

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Assay Type

Sandwich ELISA

Assay Range

12.5-800 pg/ml (example only; lot dependent)

Sensitivity

12.5 pg/ml (example only; lot dependent)

Reactivity

Mouse
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Product Specifications

Description

Solid Phase sandwich ELISA for the quantitative determination of Mouse Carboxypeptidase B1/CPB1.

Sample Volume Required

100 ul

Conjugate

HRP

Scientific Data Images for Carboxypeptidase B1/CPB1 Antibody Pair [HRP]

Sandwich ELISA: Carboxypeptidase B1/CPB1 Antibody Pair [HRP] [NBP2-79570] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Kit Contents for Carboxypeptidase B1/CPB1 Antibody Pair [HRP]

  • Rabbit Monoclonal Capture Antibody: (Catalog # NBP2-90553)
  • Rabbit Monoclonal Detection Antibody (HRP-conjugated): (Catalog # NBP2-90554)
  • Standard

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Carboxypeptidase B1/CPB1

Carboxypeptidase catalyzes the hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal position in polypeptides. It occurs as a zymogen in the pancreas in most vertebrates. This reagent is suited to enable an immunological appraoch to the study of protein and peptide structure.

Alternate Names

CPB1, PASP, PCPB

Gene Symbol

CPB1

Additional Carboxypeptidase B1/CPB1 Products

Product Documents for Carboxypeptidase B1/CPB1 Antibody Pair [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Carboxypeptidase B1/CPB1 Antibody Pair [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Related Research Areas

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FAQs for Carboxypeptidase B1/CPB1 Antibody Pair [HRP]

Showing  1 - 2 of 2 FAQs Showing All
  • Q: I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit.

    A:

    Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

  • Q: I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?

    A: Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.

  • Q: I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit.

    A:

    Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

  • Q: I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?

    A: Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.

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