Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

DyLight 350 (Excitation = 353 nm, Emission = 432 nm)

Antibody Source

Polyclonal Goat IgG
Loading...

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase B1/CPB1
His16-Tyr417
Accession # P15086

Specificity

Detects human Carboxypeptidase B1/CPB1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant mouse (rm) CPB1 is observed and less than 5% cross-reactivity with recombinant human (rh) CPA1, rhCPA2, and rmCPA4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Carboxypeptidase B1/CPB1 Antibody [DyLight 350]

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Carboxypeptidase B1/CPB1

Carboxypeptidase catalyzes the hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal position in polypeptides. It occurs as a zymogen in the pancreas in most vertebrates. This reagent is suited to enable an immunological appraoch to the study of protein and peptide structure.

Alternate Names

CPB1, PASP, PCPB

Gene Symbol

CPB1

Additional Carboxypeptidase B1/CPB1 Products

Product Documents for Carboxypeptidase B1/CPB1 Antibody [DyLight 350]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Carboxypeptidase B1/CPB1 Antibody [DyLight 350]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Customer Reviews for Carboxypeptidase B1/CPB1 Antibody [DyLight 350]

There are currently no reviews for this product. Be the first to review Carboxypeptidase B1/CPB1 Antibody [DyLight 350] and earn rewards!

Have you used Carboxypeptidase B1/CPB1 Antibody [DyLight 350]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs for Carboxypeptidase B1/CPB1 Antibody [DyLight 350]

Showing  1 - 2 of 2 FAQs Showing All
  • Q: I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit.

    A:

    Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

  • Q: I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?

    A: Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.

  • Q: I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit.

    A:

    Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

  • Q: I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?

    A: Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies
Loading...