CARD10 Antibody (HL2261) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25396

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2261 expressed in HEK293

Format

Azide and BSA Free
View all CARD10/CARMA3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human CARD10.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CARD10 Antibody (HL2261) - Azide and BSA Free

CARD10 Antibody (HL2261) - Azide and BSA Free

Western Blot: CARD10 Antibody (HL2261) - Azide and BSA Free [NBP3-25396] -

Western Blot: CARD10 Antibody (HL2261) - Azide and BSA Free [NBP3-25396] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [HL2261] (NBP3-25396) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CARD10 Antibody (HL2261) - Azide and BSA Free

Western Blot: CARD10 Antibody (HL2261) - Azide and BSA Free [NBP3-25396] -

Western Blot: CARD10 Antibody (HL2261) - Azide and BSA Free [NBP3-25396] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [HL2261] (NBP3-25396) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CARD10 Antibody (HL2261) - Azide and BSA Free

Western Blot: CARD10 Antibody (HL2261) - Azide and BSA Free [NBP3-25396] -

Western Blot: CARD10 Antibody (HL2261) - Azide and BSA Free [NBP3-25396] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [HL2261] (NBP3-25396) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CARD10 Antibody (HL2261) - Azide and BSA Free

Western Blot: CARD10 Antibody (HL2261) - Azide and BSA Free [NBP3-25396] -

Western Blot: CARD10 Antibody (HL2261) - Azide and BSA Free [NBP3-25396] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [HL2261] (NBP3-25396) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for CARD10 Antibody (HL2261) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CARD10/CARMA3

The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. CARDs induce nuclear factor kappa-B (NFKB; MIM 164011) activity through the IKK (e.g., IKBKB; MIM 603258) complex. CARD9 (MIM 607212), CARD10, CARD11 (MIM 607210), and CARD14 (MIM 607211) interact with BCL10 (MIM 603517) and are involved in NFKB signaling complexes. Except for CARD9, these CARD proteins are members of the membrane-associated guanylate kinase (MAGUK) family.

Long Name

Caspase Recruitment Domain-containing Protein 10

Alternate Names

BIMP1, CARMA3

Gene Symbol

CARD10

Additional CARD10/CARMA3 Products

Product Documents for CARD10 Antibody (HL2261) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CARD10 Antibody (HL2261) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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