CARD12 Antibody - BSA Free

Novus Biologicals | Catalog # NB500-225

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CARD12 Primary Antibodies »

Product Specifications

Immunogen

N terminus (MNFIKDNSRALIQRMGM) of the A and B isoforms of the human CLAN protein.

Reactivity Notes

This antibody reacts with human CLAN.

Localization

Cytoplasmic filaments

Specificity

Reacts with the N terminus (MNFIKDNSRALIQRMGM) of the A and B isoforms of the human CLAN protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CARD12 Antibody - BSA Free

Western Blot: CARD12 AntibodyBSA Free [NB500-225]

Western Blot: CARD12 AntibodyBSA Free [NB500-225]

CARD12-Antibody-Western-Blot-NB500-225-img0002.jpg
Western Blot: CARD12 AntibodyBSA Free [NB500-225]

Western Blot: CARD12 AntibodyBSA Free [NB500-225]

Western Blot: CARD12 Antibody [NB500-225] - Western Blot analysis of CLAN in lung lysate with anti-CLAN at a 1:500 dilution. Detects CLAN-A and CLAN-B isoforms.
CARD12 Antibody - BSA Free

Western Blot: CARD12 Antibody - BSA Free [NB500-225] -

In vitro interaction between cN-II and Ipaf B.After loading Ni-talon beads with E. coli “BL21(DE3)” crude extract without (■) or with cN-II (●), beads were washed and E. coli “BL21 Rosetta(DE3)” extracts containing Ipaf B were loaded. Elution buffer containing 250 mM imidazole was used from fraction 21 to detach His-tagged cN-II and elute proteins. A) proteins elution profile; B) immunoblotting with CARD12 and cN-II antibodies on elution fractions 3, 22, 24, 25 and 26 from chromatography.
CARD12 Antibody - BSA Free

Western Blot: CARD12 Antibody - BSA Free [NB500-225] -

Western Blot: CARD12 Antibody - BSA Free [NB500-225] - In vitro interaction between cN-II & Ipaf B.After loading Ni-talon beads with E. coli “BL21(DE3)” crude extract without (■) or with cN-II (●), beads were washed & E. coli “BL21 Rosetta(DE3)” extracts containing Ipaf B were loaded. Elution buffer containing 250 mM imidazole was used from fraction 21 to detach His-tagged cN-II & elute proteins. A) proteins elution profile; B) immunoblotting with CARD12 & cN-II antibodies on elution fractions 3, 22, 24, 25 & 26 from chromatography. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25811392), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CARD12 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:1000
Application Notes
This antibody is useful for western blot analysis where bands are seen around 110 kDa and 40 kDa representing isoforms A and B respectively.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CARD12

A novel human CED-4-like gene, CLAN, is a caspase-associated recruitment domain (CARD)-containing proteins and is expressed at varying degrees in normal human tissues. It contributes to a number of intracellular processes including apoptosis, cytokine processing, and NF-kappa B activation.

Alternate Names

CARD12leucine rich repeat and CARD domaincontaining 4, caspase recruitment domain family, member 12, Caspase recruitment domain-containing protein 12, Clan protein, CLAN1ipaf, CLANCARD, LRR, and NACHT-containing protein, CLR2.1, Ice protease-activating factor, Ipaf, NLR family, CARD domain containing 4

Entrez Gene IDs

58484 (Human)

Gene Symbol

NLRC4

Additional CARD12 Products

Product Documents for CARD12 Antibody - BSA Free

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Product Specific Notices for CARD12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CARD12 Antibody - BSA Free

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FAQs for CARD12 Antibody - BSA Free

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  • Q: What kind of blocking buffer is good for Western blot with CARD12 antibody (NB500-225)? Please tell us % tween in PBS, % milk etc.?

    A: For CARD12 antibody NB500-225, we recommend using 5% non-fat dry milk in TBST (0.1% Tween in TBS) for blocking and 1% non-fat dry milk in TBST for primary and secondary diluents. While this is the protocol we like to use, you can amend this buffer to fit your needs. We sometimes add 1% BSA to the blocking buffer for additional blocking if the blot is coming out dirty.

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