Recombinant Rat Cardiotrophin-1/CT-1 Protein

Novus Biologicals | Catalog # NBP2-35292

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Bioactivity, SDS-PAGE
View all Cardiotrophin-1/CT-1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 203 amino acids corresponding to Cardiotrophin-1/CT-1 Source: E. coli

Uniprot ID: Q63086

Amino Acid Sequence: MSQREGSLED HQTDSSFSFL PHLEAKIRQT HNLARLLTKY ADQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSALPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP VPEPVATSAL FTSNSAAGVF SAKVLGLHVC GLYGEWVSRT EGDLGQLVPG GVA

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Cardiotrophin-1/CT-1 as determined by LAL method.

Predicted Molecular Mass

21.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Cardiotrophin 1 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat Cardiotrophin-1/CT-1 Protein

SDS-PAGE: Recombinant Rat Cardiotrophin-1/CT-1 Protein [NBP2-35292]

SDS-PAGE: Recombinant Rat Cardiotrophin-1/CT-1 Protein [NBP2-35292]

SDS-Page: Rat Cardiotrophin-1/CT-1 Protein [NBP2-35292]

Formulation, Preparation, and Storage

NBP2-35292
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile, 0.1 % TFA.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in 4mM HCl to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cardiotrophin-1/CT-1

Cardiotrophin1 (CT1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a pleiotropic cytokine which is expressed in various tissues including the adult heart, skeletal muscle, ovary, colon, prostate and fetal lung. Studies showed CT-1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Rat CT1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide and it shares 94 % a.a. and 79 % a.a. sequence identity with human and murine CT-1.

Alternate Names

Cardiotrophin1, CTF1

Gene Symbol

CTF1

Additional Cardiotrophin-1/CT-1 Products

Product Documents for Recombinant Rat Cardiotrophin-1/CT-1 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Rat Cardiotrophin-1/CT-1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

IL-6 Family
Obesity

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