CASC5 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-2586

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CASC5 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 300 and 350 of human Cancer Susceptibility Candidate 5 using the numbering given in entry NP_733468.2

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CASC5 Antibody - BSA Free

Western Blot: CASC5 Antibody [NB100-2586]

Western Blot: CASC5 Antibody [NB100-2586]

CASC5-Antibody-Western-Blot-NB100-2586-img0007.jpg
Western Blot: CASC5 Antibody [NB100-2586]

Western Blot: CASC5 Antibody [NB100-2586]

Western Blot: CASC5 Antibody [NB100-2586] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CASC5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Immunoprecipitation: CASC5 Antibody [NB100-2586]

Immunoprecipitation: CASC5 Antibody [NB100-2586]

Immunoprecipitation: CASC5 Antibody [NB100-2586] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbitanti-CASC5 antibody used for IP at 6 ug per reaction. CASC5 was alsoimmunoprecipitated by a previous lot of this antibodyl. For blotting immunoprecipitated CASC5, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Applications for CASC5 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in ICC/IF was reported in scientific literature (PMID: 29497093).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CASC5

Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Four known isoforms exist.

Alternate Names

AF15q14, Blinkin, blinkin, bub-linking kinetochore protein, Bub-linking kinetochore protein, cancer susceptibility candidate 5, Cancer susceptibility candidate gene 5 protein, Cancer/testis antigen 29, CT29KIAA1570, D40, hKNL-1, hSpc105, kinetochore null 1 homolog, Kinetochore-null protein 1, KNL1, KNL1ALL1-fused gene from chromosome 15q14 protein, protein CASC5, Protein D40/AF15q14

Entrez Gene IDs

57082 (Human)

Gene Symbol

KNL1

UniProt

Q8NG31 (Human)

Additional CASC5 Products

Product Documents for CASC5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CASC5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CASC5 Antibody - BSA Free

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Protocols

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FAQs

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