Caspase-8 Activity Assay Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-54817

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell and tissue lysates
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Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Species

Human, Mouse, Rat, Mammal

Description

Simple and convenient colorimetric assay to measure Caspase-8/FLICE activity within 1-2 hrs.
    Detection method: Absorbance (400 or 405 nm)Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.


This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab39700)

Scientific Data Images for Caspase-8 Activity Assay Kit (Colorimetric)

Caspase-8 Activity Assay Kit (Colorimetric) [NBP2-54817] - The assay was performed on murine insulinoma cell line following Novu's protocol.

Kit Contents for Caspase-8 Activity Assay Kit (Colorimetric)

  • 2X Reaction Buffer
  • Cell Lysis Buffer
  • Dilution Buffer
  • DTT (1 M)
  • IETD-pNA (4 mM)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Caspase-8

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The FLICE/Caspase-8 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405 nm.

Alternate Names

CASP8, Caspase8, Mch5

Gene Symbol

CASP8

Additional Caspase-8 Products

Product Documents for Caspase-8 Activity Assay Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Caspase-8 Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Citations for Caspase-8 Activity Assay Kit (Colorimetric)

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  • Caspase-8 Activity Assay Kit (Colorimetric)
    Name: Francesca Pacifici
    Verified Customer | Posted 12/06/2018
    The assay was performed on murine insulinoma cell line by following manufacturer's protocol.
    Caspase-8 Activity Assay Kit (Colorimetric) NBP2-54817

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FAQs for Caspase-8 Activity Assay Kit (Colorimetric)

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    • Q: May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
      A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.
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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail