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Caspase-8 Activity Assay Kit (Colorimetric)
Novus Biologicals | Catalog # NBP3-50233
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Key Product Details
Sample Type & Volume Required Per Well
Cell and tissue samples
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Product Specifications
Kit Type
Activity Assay Kit (Colorimetric)
Species
Non-species specific
Description
Caspase 8, also known as FLICE, MACH and Mch5 is self-activated or activated in response to granzyme B cleavage. Caspase 8 specifically recognizes IETD. Activated caspase 8 can not only cleave PARP, but also participate in the activation of other ICE family proteases, such as caspase 3 and caspase 7. This caspase 8 activity assay kit is based on caspase 8 that can catalyze the substrate Ac-IETD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 8 can be calculated by determining the absorbance at 405 nm.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Caspase-8
Caspase 8 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 8 form.
Caspase 8 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.
Alternate Names
CASP8, Caspase8, Mch5
Gene Symbol
CASP8
Additional Caspase-8 Products
Product Documents for Caspase-8 Activity Assay Kit (Colorimetric)
Certificate of Analysis
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Product Specific Notices for Caspase-8 Activity Assay Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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FAQs for Caspase-8 Activity Assay Kit (Colorimetric)
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Q: May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.
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Associated Pathways
