Rat Caspase-9 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-75044

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat CASP9 in samples. No significant cross-reactivity or interference between Rat CASP9 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.15%

Inter-Assay Precision (Precision between assays) CV% 6.54%

Recovery for Rat Caspase-9 ELISA Kit (Colorimetric)

Recovery

85-105%

Linearity

Rat Caspase-9 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Caspase-9 ELISA Kit (Colorimetric)

Rat Caspase-9 ELISA Kit (Colorimetric)

ELISA: Rat Caspase-9 ELISA Kit (Colorimetric) [NBP2-75044] -

ELISA: Rat Caspase-9 ELISA Kit (Colorimetric) [NBP2-75044] - Standard Curve Reference

Kit Contents for Rat Caspase-9 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Caspase-9

Caspase 9 (CASP9) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. Caspase 9 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 9 form.

There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Caspase 3 is considered an initiator, as initiated caspase-9 goes on to cleave the effector procaspase-3 and procaspase-7.

Caspase 9 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Alternate Names

CASP9, Caspase9

Gene Symbol

CASP9

Additional Caspase-9 Products

Product Documents for Rat Caspase-9 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat Caspase-9 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

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FAQs for Rat Caspase-9 ELISA Kit (Colorimetric)

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  • Q: Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?

    A: After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

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